CAJ | 학술논문

目的：检测湖北地区人群Toll样受体4（TLR4）基因rs4986790和rs4986791位点单核苷酸多态性，以及与幽门螺杆菌（Hp）感染的关系。方法收集2012年12月至2013年6月在武汉大学人民医院内镜中心进行胃镜活检的就诊患者326例，同时采集患者外周血。采用免疫胶体金法检测患者外周血中 H p抗体。采用等位基因特异性聚合酶链反应检测TLR4基因rs4986790和rs4986791位点多态性。结果 TLR4基因rs4986790位点在所有研究对象中基因型均为AA型，TLR4基因rs4986791位点在所有研究对象中基因型均为CC型。结论湖北地区人群TLR4基因rs4986790和rs4986791位点不存在多态性，湖北地区人群Hp感染与rs4986790和rs49867912个位点多态性不存在相关性。
목적：검측호북지구인군Toll양수체4（TLR4）기인rs4986790화rs4986791위점단핵감산다태성，이급여유문라간균（Hp）감염적관계。방법수집2012년12월지2013년6월재무한대학인민의원내경중심진행위경활검적취진환자326례，동시채집환자외주혈。채용면역효체금법검측환자외주혈중 H p항체。채용등위기인특이성취합매련반응검측TLR4기인rs4986790화rs4986791위점다태성。결과 TLR4기인rs4986790위점재소유연구대상중기인형균위AA형，TLR4기인rs4986791위점재소유연구대상중기인형균위CC형。결론호북지구인군TLR4기인rs4986790화rs4986791위점불존재다태성，호북지구인군Hp감염여rs4986790화rs49867912개위점다태성불존재상관성。
Objective To access the the possible contribution of Toll 1ike receptor 4(TLR4) gene rs4986790 and rs4986791 polymorphism and to define association between TLR4 genotype and susceptibility of helicobacter py‐lori infection. Methods A total of 326 patients who had been examined by gastroscopy were obtained from the endos‐copy center of Renmin Hospital of Wuhan University between December 2012 and June 2013. Immune colloidal gold method was used to testing the serological Hp antibody of all the patients. The TLR4 gene rs4986790 and rs4986791 polymorphisms were examined by alleles specific polymerase chain reaction. Results All the individuals had the same TLR4 rs4986790 genotypes(AA) ,and all the individuals had the same TLR4 rs4986791 genotypes(CC). Conclusion None polymorphisms of rs4986790 and rs4986791 were detected in Hubei populations ,and showed no relationship between TLR4 genotype and susceptibility of helicobacter pylori infection.