CAJ | 학술논문

目的：筛查男性不育患者Y染色体无精子症因子（AZF）微缺失发生率，并分析缺失类型与临床表型的关系。方法选取2013年1～12月于佛山市妇幼保健院男科就诊的男性不育症患者200例，其中非梗阻性无精子症141例，重度少精子症59例；另选取100例正常孕育男性作为阳性对照；100例正常孕育女性作为阴性对照。采用聚合酶链反应分析男性Y染色体 AZF微缺失。结果特发性不育组AZF微缺失率显著高于非特发性不育组，差异有统计学意义（P＜0．05）。在所有AZF微缺失类型中以AZFc最常见，占55．6％；非梗阻性无精子症组与重度少精子症组微缺失率及微缺失类型分布情况比较，差异无统计学意义（ P＞0．05）。结论 Y 染色体 AZFa、AZFb、AZFc区微缺失与男性不育具有密切的相关性。
목적：사사남성불육환자Y염색체무정자증인자（AZF）미결실발생솔，병분석결실류형여림상표형적관계。방법선취2013년1～12월우불산시부유보건원남과취진적남성불육증환자200례，기중비경조성무정자증141례，중도소정자증59례；령선취100례정상잉육남성작위양성대조；100례정상잉육녀성작위음성대조。채용취합매련반응분석남성Y염색체 AZF미결실。결과특발성불육조AZF미결실솔현저고우비특발성불육조，차이유통계학의의（P＜0．05）。재소유AZF미결실류형중이AZFc최상견，점55．6％；비경조성무정자증조여중도소정자증조미결실솔급미결실류형분포정황비교，차이무통계학의의（ P＞0．05）。결론 Y 염색체 AZFa、AZFb、AZFc구미결실여남성불육구유밀절적상관성。
Objective To investigate incidence of Y chromosome microdeletions of azoospermia factor (AZF) in male infertility patients ,and to analyze the relationship between types of gene deletion and clinical phenotype . Methods A total of 200 cases of patients with male infertility were recruited in this study from January to December 2013 ,including 141 cases with non‐obstructive azoospermia and 59 cases with severe oligozoospermia .Another 100 healthy males were enrolled as positive control group ,and 100 healthy females were enrolled as negative control group .Male Y chromosome AZF microdeletions were analyzed by using polymerase chain reaction method .Results The incidence of AZF microdeletions of idiopathic infertility group was significantly higher than that of non‐idiopathic infertility group (P<0 .05) .In all types of AZF microdeletions ,AZFc was the most common type ,accounting for 55 .6% .No significant differences of AZF microdeletions incidence and distribution of types of AZF microdeletions were found between non‐obstructive azoospermia group and severe oligozoospermia group (P>0 .05) .Conclusion Y chromosome AZFa ,AZFb and AZFc microdeletion could be closely correlated to male infertility .