CAJ | 학술논문

目的：构建稳定表达PES1 shRNA的舌癌细胞系，研究敲低PES1基因对舌癌细胞生长的影响。方法在293 T细胞中包装并获得敲低PES1基因的病毒，然后将病毒感染Tca8113、SCC6和SCC153种舌癌细胞并筛选稳定克隆，Western印迹检测PES1蛋白的表达水平；利用生长曲线检测敲低PES1对舌癌细胞生长的影响，流式细胞术检测敲低PES1对舌癌细胞周期的影响。结果成功构建稳定表达PES1 shRNA的舌癌细胞系，敲低PES1基因能够抑制舌癌细胞的生长，使细胞周期阻滞在G0／G1期，敲低PES1抑制cyclin D1的表达。结论敲低PES1抑制舌癌细胞的生长，诱导细胞周期阻滞在G0／G1期，抑制cyclin D1的表达，因此PES1可能成为舌癌基因治疗的靶标。
목적：구건은정표체PES1 shRNA적설암세포계，연구고저PES1기인대설암세포생장적영향。방법재293 T세포중포장병획득고저PES1기인적병독，연후장병독감염Tca8113、SCC6화SCC153충설암세포병사선은정극륭，Western인적검측PES1단백적표체수평；이용생장곡선검측고저PES1대설암세포생장적영향，류식세포술검측고저PES1대설암세포주기적영향。결과성공구건은정표체PES1 shRNA적설암세포계，고저PES1기인능구억제설암세포적생장，사세포주기조체재G0／G1기，고저PES1억제cyclin D1적표체。결론고저PES1억제설암세포적생장，유도세포주기조체재G0／G1기，억제cyclin D1적표체，인차PES1가능성위설암기인치료적파표。
Objective To construct PES1 shRNA stable expression cell lines in tongue squamous cell carcinoma ( TSCC) cells and to study the effect of knockdown of PES 1 on the growth of TSCC cells .Methods Recombinant lentivirus carrying PES1 shRNA was packaged and obtained in 293T cells.TSCC cells (Tca8113, SCC6 and SCC15) were infected with the lentivirus and selected for stable cells .PES1 expression was identified by Western blot .The effect of inhibition of PES1 on the growth and cell cycle of TSCC cells was detected by growth curve and flow cytometry .Results TSCC cells stably expressing PES1 shRNA were constructed.Knockdown of PES1 inhibited cell proliferation and induced cell cycle ar-rest at G0/G1 phase.Knockdown of PES1 inhibited expression of cyclin D1 in TSCC cells.Conclusion Inhibition of PES1 results in reduced cell proliferation , cell cycle arrest at G 0/G1 phase and reduction of cyclin D 1 expression in TSCC cells . PES1 may be a target for TSCC gene therapy .