化学研究
化學研究
화학연구
CHEMICAL RESEARCHES
2015年
1期
64-69
,共6页
3-巯基丙酸%栀子甙%CdTe 量子点%荧光淬灭
3-巰基丙痠%梔子甙%CdTe 量子點%熒光淬滅
3-구기병산%치자대%CdTe 양자점%형광쉬멸
3-mercaptopropionic acid%gardenoside%CdTe quantum dots%fluorescence quenching
以3‐巯基丙酸作为稳定剂,在水溶液中合成了 CdTe 量子点;采用荧光光谱法初步研究了栀子甙对巯基丙酸稳定的 CdTe 量子点的荧光淬灭作用,考察了量子点浓度、pH 、反应时间等多种因素对量子点‐栀子甙体系荧光强度的影响,确定了测定栀子甙的最佳实验条件;并初步探讨了栀子甙与该量子点相互作用的可能反应机理.结果表明,在最佳实验条件下,巯基丙酸稳定的 CdTe 量子点对栀子甙检测的线性范围为2×10-7~4×10-6 mol/L ,检出限为1.4×10-7 mol/L ,相对标准偏差为0.355%;且常见的金属阳离子、糖类和氨基酸对栀子甙的测定无显著影响.总体而言,该方法可用于人体体液中栀子甙的检测,且两者的作用过程可初步推断为动态淬灭过程.
以3‐巰基丙痠作為穩定劑,在水溶液中閤成瞭 CdTe 量子點;採用熒光光譜法初步研究瞭梔子甙對巰基丙痠穩定的 CdTe 量子點的熒光淬滅作用,攷察瞭量子點濃度、pH 、反應時間等多種因素對量子點‐梔子甙體繫熒光彊度的影響,確定瞭測定梔子甙的最佳實驗條件;併初步探討瞭梔子甙與該量子點相互作用的可能反應機理.結果錶明,在最佳實驗條件下,巰基丙痠穩定的 CdTe 量子點對梔子甙檢測的線性範圍為2×10-7~4×10-6 mol/L ,檢齣限為1.4×10-7 mol/L ,相對標準偏差為0.355%;且常見的金屬暘離子、糖類和氨基痠對梔子甙的測定無顯著影響.總體而言,該方法可用于人體體液中梔子甙的檢測,且兩者的作用過程可初步推斷為動態淬滅過程.
이3‐구기병산작위은정제,재수용액중합성료 CdTe 양자점;채용형광광보법초보연구료치자대대구기병산은정적 CdTe 양자점적형광쉬멸작용,고찰료양자점농도、pH 、반응시간등다충인소대양자점‐치자대체계형광강도적영향,학정료측정치자대적최가실험조건;병초보탐토료치자대여해양자점상호작용적가능반응궤리.결과표명,재최가실험조건하,구기병산은정적 CdTe 양자점대치자대검측적선성범위위2×10-7~4×10-6 mol/L ,검출한위1.4×10-7 mol/L ,상대표준편차위0.355%;차상견적금속양리자、당류화안기산대치자대적측정무현저영향.총체이언,해방법가용우인체체액중치자대적검측,차량자적작용과정가초보추단위동태쉬멸과정.
CdTe quantum dots were synthesized in aqueous solution with 3‐mercaptopropionic acid as the stabilizer .The fluorescence quenching of 3‐mercaptopropionic acid stabilized CdTe quantum dots by gardenoside was investigated with fluorescence spectrometry .The effects of concentration of quan‐tum dots ,pH of buffer solution ,and reaction time on the fluorescence intensity of gardenoside/CdTe quantum dots system were examined .The optimum condition for the determination of gardenoside was established ,and the possible reaction mechanisms between gardenoside and as‐prepared CdTe quan‐tum dots were primarily studied .Results indicate that ,under the optimal experimental conditions ,the relative fluorescence intensity decreases linearly with the gardenoside concentration in the range of 2 × 10 - 7 -4 × 10 - 6 mol/L ,while the detection limit and relative standard deviation are 1 .4 × 10 - 7 mol/L and 0 .355% ,respectively .Moreover ,common metal cations ,sugars and amino acids have almost no apparent effect on the determination of gardenoside .In one word ,the present route can be readily used for the determination of gardenoside in the body fluid of human ;and the interaction between gardeno‐side and CdTe quantum dots is possibly dominated by dynamic quenching process .