CAJ | 학술논문

用5‐甲基水杨醛和对氨基水杨酸为原料，合成了一种新型席夫碱，用元素分析、IR 、1 H NMR 、MS 验证其结构。采用荧光光谱法研究该化合物与牛血清白蛋白（BSA）的相互作用机制，采用位点结合模型公式、热力学公式求解出不同温度下的结合常数、结合位点数、热力学参数，得出其淬灭机制为静态淬灭，结合位点数为1，作用力类型为氢键和范德华力。
용5‐갑기수양철화대안기수양산위원료，합성료일충신형석부감，용원소분석、IR 、1 H NMR 、MS 험증기결구。채용형광광보법연구해화합물여우혈청백단백（BSA）적상호작용궤제，채용위점결합모형공식、열역학공식구해출불동온도하적결합상수、결합위점수、열역학삼수，득출기쉬멸궤제위정태쉬멸，결합위점수위1，작용력류형위경건화범덕화력。
5‐Methyl‐salicylaldehyde‐4‐aminosalicylic acid Schiff base was obtained from 5‐meth‐yl‐salicylaldehyde and 4‐aminosalicylic acid in ethanol solution .Its structure and composition was determined through elemental analysis ,IR , 1 H NMR and MS .The interaction between Schiff base and bovine serum albumin (BSA) was studied by fluorescence spectra .The binding constants ,numbers of binding sites ,types of the binding force were found by applying the e‐quation of model of site‐binding ,thermodynamic equations at different temperatures .It is con‐cluded that the quenching mechanism is a static quenching procedure ,number of binding sites is 1 ,and types of the binding force is Van Der Waals forces and hydrogen bonding .