CAJ | 학술논문

目的：探讨辅助生殖技术中锌保护精子损伤的作用。方法：应用精子密度梯度离心的方法进行精子优选，优选的精子分为空白对照组、过氧化氢处理组和实验组，实验组受过氧化氢刺激同时加入锌，培养5h 后比较3组精子的存活率、活力、精子质膜完整性及精子 DNA 碎片。结果：过氧化氢刺激后精子的存活率、活力差，精子质膜完整性破坏，大量精子 DNA 损伤，而锌能明显保护精子质量的作用。结论：在辅助生殖技术中锌可抑制精子损伤，从而提高精子质量的作用。
목적：탐토보조생식기술중자보호정자손상적작용。방법：응용정자밀도제도리심적방법진행정자우선，우선적정자분위공백대조조、과양화경처리조화실험조，실험조수과양화경자격동시가입자，배양5h 후비교3조정자적존활솔、활력、정자질막완정성급정자 DNA 쇄편。결과：과양화경자격후정자적존활솔、활력차，정자질막완정성파배，대량정자 DNA 손상，이자능명현보호정자질량적작용。결론：재보조생식기술중자가억제정자손상，종이제고정자질량적작용。
Aim:To study the action of zinc that reduces sperm damage in assisted reproductive tech-nology.Methods:The preferred sperms selected by density gradient centrifugation were divided into blank control group,hydrogen peroxide-treated group,and experimental group which was treated with both hydrogen peroxide and Zinc;The three groups were compared in sperm motility,viability,sperm plasma membrane integrity,and sperm DNA fragmentation after 5h incubation.Results:The sperm mo-tility and vitality decreased,the membrane integrity was destroyed,and the sperm DNA was damaged af-ter the sperms were incubated with hydrogen peroxide.However,Zinc could protect the sperms from the damage significantly.Conclusion:Zinc reduces sperm damage in the assisted reproductive technology, and thereby improves the quality of sperm.