西南军医
西南軍醫
서남군의
JOURNAL OF MILITARY SURGEON IN SOUTHWEST CHINA
2015年
1期
6-9
,共4页
支气管哮喘%HLA-DQA1基因%易感基因
支氣管哮喘%HLA-DQA1基因%易感基因
지기관효천%HLA-DQA1기인%역감기인
bronchial asthma%HLA-DQA1 gene%susceptibility gene
目的：探讨人类白细胞抗原HLA-DQA1基因多态性与川南地区汉族儿童支气管哮喘的关联性，寻找与哮喘发作有关的易感基因和（或）抑制基因，为进一步研究哮喘发病机制、预防哮喘发作及寻找基因水平上治疗哮喘的方法提供相应的理论依据。方法采用聚合酶链反应-序列特异性引物（PCR-SSP）方法测定哮喘组（45例）、对照组（60例）HLA-DQAl等位基因，计算两组中各等位基因的基因频率并进行比较。结果（1）哮喘组HLA-DQA1*0301基因频率（20.00%）明显高于对照组（8.33%），P＜0.05，有统计学意义，OR为3.333（95%置信区间为1.35~8.229）。（2）哮喘组HLA-DQA1*0104,基因频率（27.77%）明显低于对照组（38.33%），P＜0.05，差异有统计学意义，OR为0.380（95%置信区间为0.164~0.880）。结论（1）HLA-DQA1*0301基因可能为儿童支气管哮喘的易感基因；（2）HLA-DQA1*0104基因可能为儿童哮喘的免疫抑制基因。
目的：探討人類白細胞抗原HLA-DQA1基因多態性與川南地區漢族兒童支氣管哮喘的關聯性，尋找與哮喘髮作有關的易感基因和（或）抑製基因，為進一步研究哮喘髮病機製、預防哮喘髮作及尋找基因水平上治療哮喘的方法提供相應的理論依據。方法採用聚閤酶鏈反應-序列特異性引物（PCR-SSP）方法測定哮喘組（45例）、對照組（60例）HLA-DQAl等位基因，計算兩組中各等位基因的基因頻率併進行比較。結果（1）哮喘組HLA-DQA1*0301基因頻率（20.00%）明顯高于對照組（8.33%），P＜0.05，有統計學意義，OR為3.333（95%置信區間為1.35~8.229）。（2）哮喘組HLA-DQA1*0104,基因頻率（27.77%）明顯低于對照組（38.33%），P＜0.05，差異有統計學意義，OR為0.380（95%置信區間為0.164~0.880）。結論（1）HLA-DQA1*0301基因可能為兒童支氣管哮喘的易感基因；（2）HLA-DQA1*0104基因可能為兒童哮喘的免疫抑製基因。
목적：탐토인류백세포항원HLA-DQA1기인다태성여천남지구한족인동지기관효천적관련성，심조여효천발작유관적역감기인화（혹）억제기인，위진일보연구효천발병궤제、예방효천발작급심조기인수평상치료효천적방법제공상응적이론의거。방법채용취합매련반응-서렬특이성인물（PCR-SSP）방법측정효천조（45례）、대조조（60례）HLA-DQAl등위기인，계산량조중각등위기인적기인빈솔병진행비교。결과（1）효천조HLA-DQA1*0301기인빈솔（20.00%）명현고우대조조（8.33%），P＜0.05，유통계학의의，OR위3.333（95%치신구간위1.35~8.229）。（2）효천조HLA-DQA1*0104,기인빈솔（27.77%）명현저우대조조（38.33%），P＜0.05，차이유통계학의의，OR위0.380（95%치신구간위0.164~0.880）。결론（1）HLA-DQA1*0301기인가능위인동지기관효천적역감기인；（2）HLA-DQA1*0104기인가능위인동효천적면역억제기인。
Objective To explore the correlation between human leukocyte antigen DQA1 gene(HLA-DQA1)polymorphisms and asthmas of han nationality children in South Sichuan so as to search out the susceptibility gene and(or)suppressor gene and to provide the theoretic basis for further study of the pathogenesis of asthma, the prevention of the occurrence of asthma and the gene therapy of asthmas. Methods Sequence specific primers-PCR(PCR-SSP)was applied in detecting HLA-DQAl alleles in 45 cases in asthma group and 60 cases in control group;the gene frequency of each allele in both group was calculated and a comparison was made. Results (1) The gene frequency of HLA-DQA1*0301 in asthma group was 20.00%, much higher than that in control group(8.33%), the difference was of statistical significance(P<0.05); OR was 3.333(95%confidence interval was 1.35-8.229);(2)The gene frequency of HLA-DQA1*0104 in asthma group was 27.77%, much lower than that in control group(38.33%), the difference was of statistical sig-nificance(P<0.05);OR was 0.380(95%confidence interval was 0.064-0.880). Conclusions(1)HLA-DQA1*0301 gene may be the susceptibility gene of childhood bronchial asthma;(2)HLA-DQA1*0104 gene may be the immune suppressor gene of childhood bron-chial asthma.
