CAJ | 학술논문

目的：分析耐甲氧西林金黄色葡萄球菌（金葡菌）（MRSA）和甲氧西林敏感金葡菌（MSSA）临床分离株基因分型及毒力基因分布特征是否存在差异，了解金葡菌耐药性演变与毒力变迁之间的相关性。方法采用脉冲场凝胶电泳（PFGE）方法和多位点序列分型（MLST）方法对呼和浩特地区住院患者中分离的30株 MRSA 和30株 MSSA 进行分子分型，同步采用聚合酶链反应（PCR）方法检测菌株毒力基因。结果60株金葡菌 PFGE 分型共分19个型，MSSA 菌株分布在 I 和 H 型等16个基因型中，而 MRSA 株主要集中分布在 K 和 M 2个基因型中。20株不同 PFGE 型菌株 MLST 分型结果显示，MRSA 株主要为 ST-239型；MSSA 株呈多样性分布特征，主要以 ST-5型、ST-7型、ST-15型为主。毒力基因在 MRSA 和 MSSA 中分布差异显著。MSSA 毒力基因整体携带率明显高于 MRSA（53．9％对40．0％，χ2＝32．7，P ＜0．01）。MRSA 株 sea、cna 和 cap 8基因携带率明显高于 MSSA 携带率（P ＜0．01），而 sec、seg 、sei、sem、sen、seo、fnbB、ebpS、cap5基因携带率明显低于 MSSA（P＜0．05）。结论呼和浩特地区金葡菌临床分离株基因型呈现多样化分布特点，MRSA 主要以 ST-239型为主。MSSA 毒力基因携带率高，特定毒力因子在 MRSA 和 MSSA 株中呈现一定聚集分布特征，金葡菌特定耐药性的获得可能伴随特定毒力特征的变化。
목적：분석내갑양서림금황색포도구균（금포균）（MRSA）화갑양서림민감금포균（MSSA）림상분리주기인분형급독력기인분포특정시부존재차이，료해금포균내약성연변여독력변천지간적상관성。방법채용맥충장응효전영（PFGE）방법화다위점서렬분형（MLST）방법대호화호특지구주원환자중분리적30주 MRSA 화30주 MSSA 진행분자분형，동보채용취합매련반응（PCR）방법검측균주독력기인。결과60주금포균 PFGE 분형공분19개형，MSSA 균주분포재 I 화 H 형등16개기인형중，이 MRSA 주주요집중분포재 K 화 M 2개기인형중。20주불동 PFGE 형균주 MLST 분형결과현시，MRSA 주주요위 ST-239형；MSSA 주정다양성분포특정，주요이 ST-5형、ST-7형、ST-15형위주。독력기인재 MRSA 화 MSSA 중분포차이현저。MSSA 독력기인정체휴대솔명현고우 MRSA（53．9％대40．0％，χ2＝32．7，P ＜0．01）。MRSA 주 sea、cna 화 cap 8기인휴대솔명현고우 MSSA 휴대솔（P ＜0．01），이 sec、seg 、sei、sem、sen、seo、fnbB、ebpS、cap5기인휴대솔명현저우 MSSA（P＜0．05）。결론호화호특지구금포균림상분리주기인형정현다양화분포특점，MRSA 주요이 ST-239형위주。MSSA 독력기인휴대솔고，특정독력인자재 MRSA 화 MSSA 주중정현일정취집분포특정，금포균특정내약성적획득가능반수특정독력특정적변화。
Objective To elucidate the difference between methicillin-resistant Staphylococcus aureus (MRSA)and methicillin-sensitive S.aureus (MSSA)in terms of genotypes and distribution of virulence genes with the clinical strains isolated from Hohhot,and explore the relationship between the changing resistance of S.aureus and the virulence transition.Methods Pulsed field gel electrophoresis (PFGE)and multi locus sequence typing (MLST)methods were employed to do molecular typing for 30 MRSA strains and 30 MSSA strains isolated from inpatients in Hohhot,Inner Mongolia.PCR method was used to profile the distribution of virulence genes among these strains.Results PFGE typing results showed that 60 S.aureus strains were classified into 19 major types.MSSA strains belonged to 16 types,mainly types I and H.MRSA strains mainly belonged to types of K and M.Among the 20 strains with different PFGE types,MRSA strains were mainly identified as ST-239 type.but the prevalence of sec ,seg ,sei,sem,sen,seo,fnbB ,ebpS and cap 5 was higher in MSSA strains than in MRSA strains (P<0.05).Conclusions The clinical strains of S .aureus isolated from Hohhot showed diverse genotyping features.ST-239 was the major PFGE type of MRSA strains.The prevalence of virulence genes was higher in MSSA strains than in MRSA strains. Characteristic cluster is found for specific virulence genes.The results also suggest that acquisition of specific antibiotic resistance may be associated with change of specific virulence feature in S.aureus.