中华胃肠外科杂志
中華胃腸外科雜誌
중화위장외과잡지
CHINESE JOURNAL OF GASTROINTESTINAL SURGERY
2015年
1期
65-68
,共4页
胃旁路术%2型糖尿病%胰岛素受体%胰岛素受体底物-1%大鼠
胃徬路術%2型糖尿病%胰島素受體%胰島素受體底物-1%大鼠
위방로술%2형당뇨병%이도소수체%이도소수체저물-1%대서
Gastric bypass%Type 2 diabetes mellitus%Insulin receptor%Insulin receptor substrate-1%Rats
目的：观察胃旁路术对2型糖尿病大鼠（GK大鼠）脂肪组织胰岛素受体β（IR-β）及胰岛素受体底物-1（IRS-1）表达的影响，探讨其改善胰岛素抵抗的机制。方法30只8周龄雄性GK大鼠（糖尿病模型）采用数字表法分为手术组（行胃旁路手术）、假手术组（与手术组大鼠相同部位切断后原位端端吻合）和饮食配对组（与手术组大鼠同种和同等质量的饮食），每组10只，另10只8周龄雄性SD大鼠作为空白对照组（自由进食及饮水）。检测术前与术后4周各组大鼠空腹血糖（FPG）和空腹胰岛素（FINS）水平，计算术前及术后4周胰岛素抵抗指数（HOMA-IR），应用蛋白印迹（Western blot）技术检测各组术后4周脂肪组织IR-β和IRS-1的表达。结果手术组术后4周FPG及 HOMA-IR 较术前明显降低（分别为5.13±0.22比11.73±0.37，2.16±0.18比5.10±0.29；均P<0.05），并能达到空白对照组术后水平（P>0.05）；而假手术组和饮食配对组较术前无显著变化（均P＞0.05）；术后4周手术组IR-β及IRS-1表达量均明显高于其他3组（均P<0.05）。结论胃旁路术能上调2型糖尿病大鼠胰岛素信号转导通路中IR-β及IRS-1的表达，改善脂肪组织胰岛素抵抗，提高胰岛素的敏感性。
目的：觀察胃徬路術對2型糖尿病大鼠（GK大鼠）脂肪組織胰島素受體β（IR-β）及胰島素受體底物-1（IRS-1）錶達的影響，探討其改善胰島素牴抗的機製。方法30隻8週齡雄性GK大鼠（糖尿病模型）採用數字錶法分為手術組（行胃徬路手術）、假手術組（與手術組大鼠相同部位切斷後原位耑耑吻閤）和飲食配對組（與手術組大鼠同種和同等質量的飲食），每組10隻，另10隻8週齡雄性SD大鼠作為空白對照組（自由進食及飲水）。檢測術前與術後4週各組大鼠空腹血糖（FPG）和空腹胰島素（FINS）水平，計算術前及術後4週胰島素牴抗指數（HOMA-IR），應用蛋白印跡（Western blot）技術檢測各組術後4週脂肪組織IR-β和IRS-1的錶達。結果手術組術後4週FPG及 HOMA-IR 較術前明顯降低（分彆為5.13±0.22比11.73±0.37，2.16±0.18比5.10±0.29；均P<0.05），併能達到空白對照組術後水平（P>0.05）；而假手術組和飲食配對組較術前無顯著變化（均P＞0.05）；術後4週手術組IR-β及IRS-1錶達量均明顯高于其他3組（均P<0.05）。結論胃徬路術能上調2型糖尿病大鼠胰島素信號轉導通路中IR-β及IRS-1的錶達，改善脂肪組織胰島素牴抗，提高胰島素的敏感性。
목적：관찰위방로술대2형당뇨병대서（GK대서）지방조직이도소수체β（IR-β）급이도소수체저물-1（IRS-1）표체적영향，탐토기개선이도소저항적궤제。방법30지8주령웅성GK대서（당뇨병모형）채용수자표법분위수술조（행위방로수술）、가수술조（여수술조대서상동부위절단후원위단단문합）화음식배대조（여수술조대서동충화동등질량적음식），매조10지，령10지8주령웅성SD대서작위공백대조조（자유진식급음수）。검측술전여술후4주각조대서공복혈당（FPG）화공복이도소（FINS）수평，계산술전급술후4주이도소저항지수（HOMA-IR），응용단백인적（Western blot）기술검측각조술후4주지방조직IR-β화IRS-1적표체。결과수술조술후4주FPG급 HOMA-IR 교술전명현강저（분별위5.13±0.22비11.73±0.37，2.16±0.18비5.10±0.29；균P<0.05），병능체도공백대조조술후수평（P>0.05）；이가수술조화음식배대조교술전무현저변화（균P＞0.05）；술후4주수술조IR-β급IRS-1표체량균명현고우기타3조（균P<0.05）。결론위방로술능상조2형당뇨병대서이도소신호전도통로중IR-β급IRS-1적표체，개선지방조직이도소저항，제고이도소적민감성。
Objective To investigate the expressions of adipic insulin receptor β (IR-β) and insulin receptor substrate-1 (IRS-1) after gastric bypass (GBP) operation in spontaneous rats with type 2 diabetes mellitus (GK rats) and to elucidate the mechanisms of GBP in improving insulin resistance. Methods Thirty male GK rats aged 8 weeks were randomly divided into 3 groups according to the table of random number:the operation group (GBP, 10 rats), the sham operation group (the same sites were cut off as GBP and end to end anastomosis was performed in site, 10 rats) and the diet pairing group (the same kind and weight dieting as the operation group, 10 rats), besides 10 male SD rats aged 8 weeks were used as blank control group (free eating and drinking). Four weeks before and after operation, levels of fasting blood glucose (FPG) and fasting insulin (FINS) were measured, HOMA-IR was calculated respectively, and compared among 4 groups. Then rats were decapitated to retrieve the omentum. Expressions of adipic IR-βand IRS-1 protein were detected by Western blot. Results Compared with the preoperative levels, the FPG and HOMA-IR decreased significantly 4 weeks after surgery in operation group [(5.13±0.22) vs. (11.73±0.37) mmol/L, 2.16± 0.18 vs. 5.10±0.29, P<0.05), reaching the level of blank control group(P>0.05). FINS showed no obvious change in these 4 groups after operation(all P>0.05). Expressions of IR-β and IRS-1 were significantly higher in operation group than those in other 3 groups 4 weeks after the operation (all P<0.05) . Conclusions Expressions of adipic IR-βand IRS-1 in insulin signal transmission of rats with type 2 diabetes mellitus after GBP are up-regulated , meanwhile insulin resistance can be improved and insulin sensibility increases.
