功能与分子医学影像学杂志（电子版）
功能與分子醫學影像學雜誌（電子版）
공능여분자의학영상학잡지（전자판）
Functional and Molecular Medical Imaging(Electronic Edition)
2014年
4期
510-516
,共7页
吴富淋%陈秋雁%缪绍维%魏鼎泰
吳富淋%陳鞦雁%繆紹維%魏鼎泰
오부림%진추안%무소유%위정태
肢体远端缺血预处理%脑卒中%水肿%水通道蛋白-4%诱导型一氧化氮合酶%DWI
肢體遠耑缺血預處理%腦卒中%水腫%水通道蛋白-4%誘導型一氧化氮閤酶%DWI
지체원단결혈예처리%뇌졸중%수종%수통도단백-4%유도형일양화담합매%DWI
limb remote ischemic preconditioning%stroke%edema%AQP4%iNOS%DWI
目的：探讨经肢体远端缺血预处理（LRP）后大鼠脑缺血模型的弥散加权成像（DWI）改变及其相关机制。方法98只成年SD大鼠随机分为三组，LRP组、缺血对照组及假手术组。分别在恢复再灌后1?h、3?h、12?h及24?h行MRI检查采集T2WI/DWI图像；并结合神经学评分、TTC染色从行为学和形态学上进行辅助评价。Western?blot检测和免疫荧光检测缺血组和LRP组各时间点，及假手术组AQP4、iNOS的表达情况，并通过共染进行定位。结果与另外两组比较LRP组行为学评分增加，大脑梗死面积减少，同时LRP组在DWI上出现的病灶亦较缺血对照组减小。连续性的DWI检测显示LRP组的病灶出现在恢复再灌后3?h，而缺血对照组多出现在1?h。Western?blot表明AQP4和CGRP均在缺血后表达下降，而LRP组的AQP4表达水平则高于假手术组，并有随着时间的延长而上升的趋势。iNOS在缺血后的表达量较假手术组增多，而LRP组表达降低。AQP4主要表达在血管内皮细胞上及邻近血管周围的细胞膜上。结论 LRP减轻脑缺血引起的脑水肿很可能与上调AQP4的表达及抑制iNOS的表达相关。
目的：探討經肢體遠耑缺血預處理（LRP）後大鼠腦缺血模型的瀰散加權成像（DWI）改變及其相關機製。方法98隻成年SD大鼠隨機分為三組，LRP組、缺血對照組及假手術組。分彆在恢複再灌後1?h、3?h、12?h及24?h行MRI檢查採集T2WI/DWI圖像；併結閤神經學評分、TTC染色從行為學和形態學上進行輔助評價。Western?blot檢測和免疫熒光檢測缺血組和LRP組各時間點，及假手術組AQP4、iNOS的錶達情況，併通過共染進行定位。結果與另外兩組比較LRP組行為學評分增加，大腦梗死麵積減少，同時LRP組在DWI上齣現的病竈亦較缺血對照組減小。連續性的DWI檢測顯示LRP組的病竈齣現在恢複再灌後3?h，而缺血對照組多齣現在1?h。Western?blot錶明AQP4和CGRP均在缺血後錶達下降，而LRP組的AQP4錶達水平則高于假手術組，併有隨著時間的延長而上升的趨勢。iNOS在缺血後的錶達量較假手術組增多，而LRP組錶達降低。AQP4主要錶達在血管內皮細胞上及鄰近血管週圍的細胞膜上。結論 LRP減輕腦缺血引起的腦水腫很可能與上調AQP4的錶達及抑製iNOS的錶達相關。
목적：탐토경지체원단결혈예처리（LRP）후대서뇌결혈모형적미산가권성상（DWI）개변급기상관궤제。방법98지성년SD대서수궤분위삼조，LRP조、결혈대조조급가수술조。분별재회복재관후1?h、3?h、12?h급24?h행MRI검사채집T2WI/DWI도상；병결합신경학평분、TTC염색종행위학화형태학상진행보조평개。Western?blot검측화면역형광검측결혈조화LRP조각시간점，급가수술조AQP4、iNOS적표체정황，병통과공염진행정위。결과여령외량조비교LRP조행위학평분증가，대뇌경사면적감소，동시LRP조재DWI상출현적병조역교결혈대조조감소。련속성적DWI검측현시LRP조적병조출현재회복재관후3?h，이결혈대조조다출현재1?h。Western?blot표명AQP4화CGRP균재결혈후표체하강，이LRP조적AQP4표체수평칙고우가수술조，병유수착시간적연장이상승적추세。iNOS재결혈후적표체량교가수술조증다，이LRP조표체강저。AQP4주요표체재혈관내피세포상급린근혈관주위적세포막상。결론 LRP감경뇌결혈인기적뇌수종흔가능여상조AQP4적표체급억제iNOS적표체상관。
ObjectiveTo?investigate?the?changes?in?DWI?images?ofrats?that?underwent?limb?remote?ischemic?preconditioning?and?investigate?the?possible?mechanism. MethodAdult?SD?male?rats?were?subjected?to?limb?remote?ischemic?preconditioning(LRP),?control?ischemia?and?sham?surgery,?animals?then?underwent?MR?T2WI/DWI?imaging?1?h,?3?h,?12?h,?24?h?after?restore?reperfusion.?After?that?neurological?behavior?evaluation?and?TTC?staining?were?carried?out.?Western?blot?and?immunofluorescence?staining?were?used?to?investigate?the?expression?of?AQP4?and?iNOS. ResultLimb?remote?ischemic?preconditioning?improved?the?neurologic?deficit?score,?Infarct?size?was?reduced,?the?lesions?on?the?DWI?were?also?reduced?compared?to?the?control?ischemia?group.?Such?results?were?consistent?with?the?result?of?MRI?detection.?Results?of?successional?DWI?detection?showed?the?presence?of?lesion?was?prolonged?by?limb-preconditioning?from?1?hour?after?in?control?ischemia?to?3?hour. Western?blot?and?immunostaining?analysis?showed?that?LRP?accelerated?protein?expression?of?both?AQP4?wich?were?decreased?after?stroke.In?addition,?LRP?decreased?iNOS?expression?after?stoke. ConclusionLimb?remote?preconditioning?performed?in?one?limb?may?alleviate?encephaledema?leaded?by?ischemia?by?accelerating?the?expression?of?AQP4?and?restricting?the?expression?of?iNOS.
