中国医学创新
中國醫學創新
중국의학창신
MEDICAL INNOVATION OF CHINA
2014年
34期
29-31
,共3页
免疫性血小板减少症%CD28%CD80%CD86
免疫性血小闆減少癥%CD28%CD80%CD86
면역성혈소판감소증%CD28%CD80%CD86
Immune thrombocytopenia%CD28%CD80%CD86
目的：探讨特发性血小板减少性紫癜（ITP）患者外周血淋巴细胞CD28、CD80及CD86的表达及意义。方法：采用免疫荧光标记和流式细胞术检测30例ITP患者和30例健康对照者外周血CD28细胞、CD80细胞和CD86细胞分别占淋巴细胞的比例及血小板表面相关抗体水平，并进行对比、分析。结果：与正常对照组相比，ITP患者外周血CD28细胞增多，差异有统计学意义（P<0.05）；CD80细胞增多，但差异无统计学意义（P>0.05）；CD86细胞显著增多，差异有统计学意义（P<0.05），此结果与ITP是否急慢性无关。治疗前后患者的CD28、CD80及CD86的表达率比较差异无统计学意义（P>0.05）。患者与正常对照组PAIgA、PAIgG和PAIgM的水平比较差异有统计学意义（P<0.05）；CD80、CD86表达与PAIgG水平之间存在显著的相关性，比较差异有统计学意义（P<0.01）。结论：ITP患者外周血淋巴细胞CD28、CD80及CD86表达均异常，可能与其发病相关。
目的：探討特髮性血小闆減少性紫癜（ITP）患者外週血淋巴細胞CD28、CD80及CD86的錶達及意義。方法：採用免疫熒光標記和流式細胞術檢測30例ITP患者和30例健康對照者外週血CD28細胞、CD80細胞和CD86細胞分彆佔淋巴細胞的比例及血小闆錶麵相關抗體水平，併進行對比、分析。結果：與正常對照組相比，ITP患者外週血CD28細胞增多，差異有統計學意義（P<0.05）；CD80細胞增多，但差異無統計學意義（P>0.05）；CD86細胞顯著增多，差異有統計學意義（P<0.05），此結果與ITP是否急慢性無關。治療前後患者的CD28、CD80及CD86的錶達率比較差異無統計學意義（P>0.05）。患者與正常對照組PAIgA、PAIgG和PAIgM的水平比較差異有統計學意義（P<0.05）；CD80、CD86錶達與PAIgG水平之間存在顯著的相關性，比較差異有統計學意義（P<0.01）。結論：ITP患者外週血淋巴細胞CD28、CD80及CD86錶達均異常，可能與其髮病相關。
목적：탐토특발성혈소판감소성자전（ITP）환자외주혈림파세포CD28、CD80급CD86적표체급의의。방법：채용면역형광표기화류식세포술검측30례ITP환자화30례건강대조자외주혈CD28세포、CD80세포화CD86세포분별점림파세포적비례급혈소판표면상관항체수평，병진행대비、분석。결과：여정상대조조상비，ITP환자외주혈CD28세포증다，차이유통계학의의（P<0.05）；CD80세포증다，단차이무통계학의의（P>0.05）；CD86세포현저증다，차이유통계학의의（P<0.05），차결과여ITP시부급만성무관。치료전후환자적CD28、CD80급CD86적표체솔비교차이무통계학의의（P>0.05）。환자여정상대조조PAIgA、PAIgG화PAIgM적수평비교차이유통계학의의（P<0.05）；CD80、CD86표체여PAIgG수평지간존재현저적상관성，비교차이유통계학의의（P<0.01）。결론：ITP환자외주혈림파세포CD28、CD80급CD86표체균이상，가능여기발병상관。
Objective:To investigate the expression and significance of costimulatory on peripheral lymphocytes of CD28,CD80 and CD86 in patients with immune thrombocytopenia(ITP).Method:Blood samples were collected from 30 ITP patients and 30 healthy controls.The percentages of CD28,CD80 and CD86 on lymphocytes and platelet associated immunoglobulins(PAIg)were compared and anzlysed with flow cytometry and monoclonal antibodies technology. Result:The percentage of CD28 was higher in ITP patients than those in lontrols,the difference was statistically significant(P<0.05).higher proportions of CD80 in ITP patients than those in controls,but there was not statistically significant(P>0.05).The percentage of CD86 in ITP patients was higher than in controls,this result had nothing to do with ITP whether acute or chronic.Before and after treatment in patients with the expression of CD28,CD80 and CD86 had no statistically significant differences(P>0.05).The levels of PAIgA,PAIgG and PAIgM in ITP patients were higher than those in controls. The expression of CD80 and CD86 had positive correlation with the level of PAIgG,the differences were statistically significant(P<0.01).Conclusion:The abnormal expression of costimulatory on peripheral lymphocytes of CD28,CD80 and CD86 in patients may be related to the pathogenesis of ITP.
