CAJ | 학술논문

目的：对双歧杆菌属编码的Ⅱ类毒素-抗毒素（TA）系统MazEF进行分子进化分析。方法：利用PSI-BLAST在NCBI中搜索获得双歧杆菌编码的MazF分子序列，在NCBI蛋白数据库、核酸数据库分别下载其他菌属基因组编码的MazF蛋白序列和本研究所涉及菌属的16 S rRNA序列，应用Clustal X2和MEGA4软件对MazF做分子进化分析。结果：共搜索到双歧杆菌株染色体编码毒素蛋白MazF 26个。分子进化分析显示：整个双歧杆菌属的MazF保守性不好；MazF与16 S rRNA的共进化仅在个别菌种中发现，大部分MazF的聚类结果与相应菌种16 S rRNA的聚类结果不同。结论：双歧杆菌中的TA系统MazEF可能是通过基因的水平转移整合入基因组中，属于菌株非核心基因组部分。
목적：대쌍기간균속편마적Ⅱ류독소-항독소（TA）계통MazEF진행분자진화분석。방법：이용PSI-BLAST재NCBI중수색획득쌍기간균편마적MazF분자서렬，재NCBI단백수거고、핵산수거고분별하재기타균속기인조편마적MazF단백서렬화본연구소섭급균속적16 S rRNA서렬，응용Clustal X2화MEGA4연건대MazF주분자진화분석。결과：공수색도쌍기간균주염색체편마독소단백MazF 26개。분자진화분석현시：정개쌍기간균속적MazF보수성불호；MazF여16 S rRNA적공진화부재개별균충중발현，대부분MazF적취류결과여상응균충16 S rRNA적취류결과불동。결론：쌍기간균중적TA계통MazEF가능시통과기인적수평전이정합입기인조중，속우균주비핵심기인조부분。
Objective:To perform molecular evolution analysis of MazEF in genomes of Bifidobacterium. Method:The Protein sequences of MazF in bifidobacterial genomes were obtained by employing the PSI-BLAST search from NCBI. The Protein sequences of MazF encoded by other bacteria were obtained from non-redundant protein database of NCBI. All of the 16 S rRNA sequences used in this study were obtained from non-redundant nucleotide database of NCBI. Clustal χ2 and MEGA4 were used to analyze molecular evolution of MazF. Result:The total number of MazF in bifidobacterial genomes were searched of 26 The molecular evolution analysis showed that MazF in bifidobacterial genomes had less conservation;the co-evolution of MazF with the 16 S RNA was only observed in individual species and most homologous analysis results of MazF were not in concordance with 16 S rRNA. Conclusion:MazEF was integrated in bifidobacterial chromosomes through horizontal transfer,which was a part of the non-core genome.