CAJ | 학술논문

目的：该研究旨在探讨谷胱甘肽S-转移酶基因（GST）M1、T1及P1基因多态性与特发性男性不育症的相关性。方法该病例-对照研究包括246例特发性少弱精子症男性不育患者及117例正常健康有生育史男性对照。采用聚合酶链反应（PCR）、聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法分别对GSTM1、GSTT1和GSTP1基因进行分型。结果 GSTM1基因缺失型在正常对照组和特发性少弱精子症不育患者组基因型频率分别为41.88%和60.57%，具有显著差异（P=0.001）；GST T1基因缺失型在正常对照组和特发性少弱精子症不育患者组基因型频率分别为47.86%和62.60%，具有显著差异（P=0.008）；GSTM1/T1基因缺失型在正常对照组和特发性少弱精子症不育患者组基因型频率分别为14.53%和38.62%，具有显著差异（P＜0.001）；GSTP1基因突变型在正常对照组和特发性少弱精子症不育患者组基因型频率分别为27.35%和32.11%，无明显差异（P=0.847）。结论GSTM1、GSTT1基因缺失型分别是特发性男性不育症的危险因素。GSTP1基因突变型与特发性男性不育症患者无明显相关性。
목적：해연구지재탐토곡광감태S-전이매기인（GST）M1、T1급P1기인다태성여특발성남성불육증적상관성。방법해병례-대조연구포괄246례특발성소약정자증남성불육환자급117례정상건강유생육사남성대조。채용취합매련반응（PCR）、취합매련반응-한제성편단장도다태성（PCR-RFLP）방법분별대GSTM1、GSTT1화GSTP1기인진행분형。결과 GSTM1기인결실형재정상대조조화특발성소약정자증불육환자조기인형빈솔분별위41.88%화60.57%，구유현저차이（P=0.001）；GST T1기인결실형재정상대조조화특발성소약정자증불육환자조기인형빈솔분별위47.86%화62.60%，구유현저차이（P=0.008）；GSTM1/T1기인결실형재정상대조조화특발성소약정자증불육환자조기인형빈솔분별위14.53%화38.62%，구유현저차이（P＜0.001）；GSTP1기인돌변형재정상대조조화특발성소약정자증불육환자조기인형빈솔분별위27.35%화32.11%，무명현차이（P=0.847）。결론GSTM1、GSTT1기인결실형분별시특발성남성불육증적위험인소。GSTP1기인돌변형여특발성남성불육증환자무명현상관성。
ObjectiveTo investigate the relationship between glutathione S- transferase enzyme gene GSTM1, T1 and P1 gene polymorphism and idiopathic male infertility.Methods Total of 246 patients with idiopathic male infertility and 117 healthy normal control were enrolled in the case-control study. Polymerase chain reaction (PCR) and polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) were used here to identify the genotype of GSTM1, GSTT1 and GSTP1. Results The frequency of glutathione S- transferase M1 (-)genotype in the normal control group and idiopathic male infertility were 41.88% and 60.57%, with significant difference (P=0.001); and the frequency of glutathione S-transferase T1(-)genotype in the normal control group and idiopathic male infertility patients were 47.86% and 62.60%, with significant difference (P=0.008); and the frequency of glutathione S- transferase M1/T1 (-/-) genotype in the normal control group and idiopathic male infertility patients were 14.53% and 38.62%, with significant difference (P<0.001); however the frequencies of glutathione S- transferase P1 gene mutation type in normal control group and idiopathic male infertility patients were 27.35% and 32.11%, with no significant difference (P=0.847).Conclution This study suggestd that GSTM1, GSTT1 gene deletion type may be the risk factors of idiopathic male infertility, but GSTP1 gene mutation types is not associated with idiopathic male infertility.