CAJ | 학술논문

目的：探讨 Rv0222／ESAT-6融合蛋白对结核病的血清学诊断价值。方法：表达、纯化重组 Rv0222／ESAT-6融合蛋白，以其为抗原包板、以酶标抗体为二抗、采用酶联免疫吸附试验（ELISA）检测结核患者、人类免疫缺陷病毒（human immunodefi-ciency virus，HIV）感染合并结核患者、非结核患者以及正常人血清，评价该融合蛋白对结核病的诊断价值。结果：以结核分枝杆菌感染 T 淋巴细胞斑点试验（T-SPOT，简称 T-SPOT）为金标准，将 Rv0222／ESAT-6融合蛋白作为抗原的 ELISA 诊断结核病的敏感性为86％，特异性为100％；T-SPOT 与 ELISA 对结核病的阳性检出率差异无统计学意义。结论：以 Rv0222／ES-AT-6融合蛋白作为抗原的 ELISA 对结核病（包括 HIV 合并结核病）的血清学诊断有一定价值。
목적：탐토 Rv0222／ESAT-6융합단백대결핵병적혈청학진단개치。방법：표체、순화중조 Rv0222／ESAT-6융합단백，이기위항원포판、이매표항체위이항、채용매련면역흡부시험（ELISA）검측결핵환자、인류면역결함병독（human immunodefi-ciency virus，HIV）감염합병결핵환자、비결핵환자이급정상인혈청，평개해융합단백대결핵병적진단개치。결과：이결핵분지간균감염 T 림파세포반점시험（T-SPOT，간칭 T-SPOT）위금표준，장 Rv0222／ESAT-6융합단백작위항원적 ELISA 진단결핵병적민감성위86％，특이성위100％；T-SPOT 여 ELISA 대결핵병적양성검출솔차이무통계학의의。결론：이 Rv0222／ES-AT-6융합단백작위항원적 ELISA 대결핵병（포괄 HIV 합병결핵병）적혈청학진단유일정개치。
Objective:To explore the serodiagnostic value of Rv0222/ESAT-6 fusion protein in tuberculosis.Methods:By using purified recombinant Rv0222/ESAT-6 fusion protein as antigen and enzyme-labeled antibody as secondary antibody,the serum of patients with tuberculosis and patients with human immunodeficiency virus(HIV)accompanied by tuberculosis,non-tubercu-losis patients and healthy people were detected with enzyme-linked immunosorbent assay(ELISA).The diagnostic value of the fusion protein in tuberculosis was evaluated.Results:By setting T lymphocyte spot test of infection with Mycobacterium tuber-culosis (T-SPOT)as the gold standard,the sensitivity and specificity of ELISA,of which Rv0222/ESAT-6 fusion protein was used as the antigen,in the diagnosis of tuberculosis were 86% and 100%,respectively.There was no statistically significant difference in the positive rate of tuberculosis between T-SPOT and ELISA.Conclusions:The ELISA with Rv0222/ESAT-6 fu-sion protein as antigen,has certain application value in the serodiagnosis of tuberculosis.