CAJ | 학술논문

目的：探讨采用18F-FDG直接标记含KLVFF序列的寡肽的方法。方法按常规制备18F-FDG，然后用其与上述寡肽进一步反应进行标记，分别采用不同pH值、不同比活度的18F-FDG、无修饰及氨基端HYNIC修饰的寡肽进行反应，分别比较标记产率。结果 pH值和18F-FDG比活度值的变化，及寡肽氨基端是否有HYNIC修饰对标记产率均有明显影响，pH值为3.0，18F-FDG比活度值为37.0?MBq/ml，在GKLVFFGK末端连接HYNIC后标记产率最高（35﹪），产物放射化学纯度达到98﹪。结论采用18F-FDG与氨基端HYNIC修饰的含KLVFF序列的寡肽具有可行性，能获得高标记产率，其合成方法可满足进一步研究和临床应用的需要。
목적：탐토채용18F-FDG직접표기함KLVFF서렬적과태적방법。방법안상규제비18F-FDG，연후용기여상술과태진일보반응진행표기，분별채용불동pH치、불동비활도적18F-FDG、무수식급안기단HYNIC수식적과태진행반응，분별비교표기산솔。결과 pH치화18F-FDG비활도치적변화，급과태안기단시부유HYNIC수식대표기산솔균유명현영향，pH치위3.0，18F-FDG비활도치위37.0?MBq/ml，재GKLVFFGK말단련접HYNIC후표기산솔최고（35﹪），산물방사화학순도체도98﹪。결론채용18F-FDG여안기단HYNIC수식적함KLVFF서렬적과태구유가행성，능획득고표기산솔，기합성방법가만족진일보연구화림상응용적수요。
ObjectiveTo?investigate?the?feasibility?of??directly?radiolabeling?GKLVFFGK?peptide?with18F-FDG. MethodsAfter?thesynthesis?of?18F-FDG?with?conventional?protocol,?radiolabeling?of?the?GKLVFFGK?peptide?was?performed?under?the?pH?value(3.0?and?7.0),?with?different?18F-FDG?specific?activity(11.0?and?30.0?MBq/mL),?and?with?nick?GKLVFFGK?as?well?as?the?6-hydrazine?nicotinamide(HYNIC)modified?GKLVFFGK?in-NH2?terminal. Resultsthe?changes?of?pH,?18F-FDG?specific?activity?and?GKLVFFGK?structure?has?obvious?effect?on?the?radiochemical?yield?of?18F-FDG?GKLVFFGK.?The?yield?of?the?reaction?using?HYNIC-GKLVFFGK?with?a?pH?value?of?3.0?and?a?18F-FDG?specific?activity?of?37.0MBq/mlis?the?highest(35﹪). ConclusionIt?is?feasible?to?achieve?high?yield?by?employingthe?direct?radiolabeling?with?18F-FDG?and?HYNIC-GKLVFFGK.?This?method?can?meet?the?requirement?of?the?scientific?research?and?clinical?application.