CAJ | 학술논문

目的：对大鼠肝脏纤维化模型行13N-NH3?H2O?PET/CT和多b值DWI对比研究，探讨不同b值DWI对肝脏纤维化早期诊断及分期的价值。方法15只大鼠，对照组3只，肝脏纤维化模型12只（S1期、S2期和S3期分别是6只、5只和1只）。15只大鼠均先做13N-NH3?H2O?PET/CT扫描，然后进行常规MR和多b值扫描。扫描结束后取出肝脏标本进行常规病理和水通道蛋白1（AQP1）免疫组化分析。获得13N-NH3?H2O?PET/CT?20?s、90?s、5?min、10?min和15?min肝脏标准摄取值（SUV），及单指数模型、多指数模型ADC的平均值，然后计算不同时相SUV和ADC值之间的相关系数。结果13N-NH3?H2O?PET/CT?90?s时对照组和S1组、5?min时对照组和S2?+?S3组，及多指数模型对照组和S2?+?S3组的中b值和高b值ADC值差异有统计学意义，其余各组之间差异均无统计学意义。单指数模型ADC值与13N-NH3?H2O?PET/CT肝脏在90?s的SUV相关性最高，多指数模型小b值、中b值和高b值ADC值分别与13N-NH3?H2O?PET/CT肝脏在20?s、5?min和15?min的SUV相关性最高。结论13N-NH3?H2O?PET/CT肝脏SUV和ADC值并不能区分对照组和S1组、S1和S2?+?S3分期。ADC值与13N-NH3?H2O?PET?SUV的相关性表明，单指数模型ADC值与水分子扩散密切相关，多指数模型小b值、中b值分别与组织血流灌注、细胞间隙和细胞内水分子扩散高度相关，而高b值与水分子通过细胞膜转运相关。
목적：대대서간장섬유화모형행13N-NH3?H2O?PET/CT화다b치DWI대비연구，탐토불동b치DWI대간장섬유화조기진단급분기적개치。방법15지대서，대조조3지，간장섬유화모형12지（S1기、S2기화S3기분별시6지、5지화1지）。15지대서균선주13N-NH3?H2O?PET/CT소묘，연후진행상규MR화다b치소묘。소묘결속후취출간장표본진행상규병리화수통도단백1（AQP1）면역조화분석。획득13N-NH3?H2O?PET/CT?20?s、90?s、5?min、10?min화15?min간장표준섭취치（SUV），급단지수모형、다지수모형ADC적평균치，연후계산불동시상SUV화ADC치지간적상관계수。결과13N-NH3?H2O?PET/CT?90?s시대조조화S1조、5?min시대조조화S2?+?S3조，급다지수모형대조조화S2?+?S3조적중b치화고b치ADC치차이유통계학의의，기여각조지간차이균무통계학의의。단지수모형ADC치여13N-NH3?H2O?PET/CT간장재90?s적SUV상관성최고，다지수모형소b치、중b치화고b치ADC치분별여13N-NH3?H2O?PET/CT간장재20?s、5?min화15?min적SUV상관성최고。결론13N-NH3?H2O?PET/CT간장SUV화ADC치병불능구분대조조화S1조、S1화S2?+?S3분기。ADC치여13N-NH3?H2O?PET?SUV적상관성표명，단지수모형ADC치여수분자확산밀절상관，다지수모형소b치、중b치분별여조직혈류관주、세포간극화세포내수분자확산고도상관，이고b치여수분자통과세포막전운상관。
Objective To?compare?the?13N-NH3H2O?PET/CT?and?multi-b?DWI?images?of?rat?liver?fibrosis?model?and?to?explore?the?value?of?different?b?values?in?DWI?in?the?early?diagnosis?and?staging?of?liver?fibrosis.?Methods 13N-NH3H2O?PET/CT?and?multi-b?DWI?scan?were?performed?on?15?rats,?including?a?control?group(n?=?3),?and?a?group?of?rat?with?liver?fibrosis?of?various?stages(n?=?12?including?6?at?S1,?5?at?S2?and?1?at?S3).?After?that?routine?pathologic?analysis?was?performed?on?liver?specimens?and?the?aquaporin1(AQP1)?immunohistochemical?information?was?obtained.?Liver?standardized?uptake?value(SUV)were?obtained?from?13N-NH3H2O?PET/CT?in?20?seconds,?90?seconds,?5?minutes,?10?minutes?and?15?minutesrespectively;?and?ADC?from?single?index?model?and?multi-exponential?model?were?averaged.?The?correlation?between?the?SUV?values?and?ADC?were?calculated.?Results There?are?not?significant?difference?among?control,?S1?and?S2?+?S3?group?excluding?13N-NH3H2O?PET/CT?between?control?and?S1?group?at?90?s,?between?control?and?S2?+?S3?group?at?5min,?multi-index?model?ADC?of??middle?b?and?high?b?value?between?control?and?S2?+?S3?group?respectively.?ADC?from?single?index?model?and?the?SUV?from?13N-NH3H2O?PET/CT?of?the?liver?in?90?s?shows?the?highest?correlation,?ADC?values?from?multi-index?model?of?low?b,?middle?b;?high?b?values?correlated?with?SUV?from?13N-NH3H2O?PET/CT?in?20?s,?15?min?and?5?min?the?best.?Conclusion 13N-NH3H2O?PET/CT?liver?SUV?and?ADC?do?not?distinguish?between?control?and?S1?group,?between?S1?and?S2?+?S3?group.?Our?results?showed?that?ADC?values?from?single?index?model?is?closely?related?to?the?diffusion?of?water?molecules;?ADC?values?from?multi-index?of?low?b,?middle?b?and?high?b?value?correlated?with?in?tissue?perfusion,?molecular?diffusion?in?cell?and?tissue?waters?respectively;?and?high?b?values?associated?with?the?water?molecules?through?the?membrane?transport.