CAJ | 학술논문

本文旨在探究移植脐带间充质干细胞( UC-MSCs)对荷斯坦公犊体重及血清免疫和抗氧化水平的影响。选取1月龄体重相近的荷斯坦公犊28头,随机分为对照组和试验组,每组14头。采集健康的荷斯坦新生胎牛的脐带组织,通过胰酶消化筛选法体外分离、纯化培养UC-MSCs,试验组公犊颈静脉注射UC-MSCs 3×105个/kg BW(稀释至8 mL),对照组注射等量生理盐水,分别与1、2、3、4、5月龄各注射1次。试验期150 d。结果表明：与对照组相比,公犊移植UC-MSCs,1)显著提高4、5、6月龄体重( P<0.05)；显著提高3、4月龄( P<0.05),极显著提高6月龄(P<0.01)的平均日增重；2)极显著提高4月龄(P<0.01),显著提高6月龄(P<0.05)血清免疫球蛋白A(IgA)含量；极显著提高2、5月龄(P<0.01),显著提高6月龄(P<0.05)血清免疫球蛋白G(IgG)含量；极显著降低3月龄(P<0.01),显著降低4月龄(P<0.05)血清白细胞介素2( IL-2)含量；3)极显著降低2、3、4、5、6月龄血清丙二醛( MDA)含量( P<0.01)；显著提高5月龄( P<0.05),极显著提高6月龄( P<0.01)血清超氧化物歧化酶( SOD)活性。由此可见,移植UC-MSCs能够提高公犊血清IgA、IgG含量；降低血清MDA含量,提高SOD活性,增强机体清除自由基的能力；降低血清IL-2含量,减少内部炎症发生；从而提高公犊机体免疫力,进而促进公犊的生长发育。
본문지재탐구이식제대간충질간세포( UC-MSCs)대하사탄공독체중급혈청면역화항양화수평적영향。선취1월령체중상근적하사탄공독28두,수궤분위대조조화시험조,매조14두。채집건강적하사탄신생태우적제대조직,통과이매소화사선법체외분리、순화배양UC-MSCs,시험조공독경정맥주사UC-MSCs 3×105개/kg BW(희석지8 mL),대조조주사등량생리염수,분별여1、2、3、4、5월령각주사1차。시험기150 d。결과표명：여대조조상비,공독이식UC-MSCs,1)현저제고4、5、6월령체중( P<0.05)；현저제고3、4월령( P<0.05),겁현저제고6월령(P<0.01)적평균일증중；2)겁현저제고4월령(P<0.01),현저제고6월령(P<0.05)혈청면역구단백A(IgA)함량；겁현저제고2、5월령(P<0.01),현저제고6월령(P<0.05)혈청면역구단백G(IgG)함량；겁현저강저3월령(P<0.01),현저강저4월령(P<0.05)혈청백세포개소2( IL-2)함량；3)겁현저강저2、3、4、5、6월령혈청병이철( MDA)함량( P<0.01)；현저제고5월령( P<0.05),겁현저제고6월령( P<0.01)혈청초양화물기화매( SOD)활성。유차가견,이식UC-MSCs능구제고공독혈청IgA、IgG함량；강저혈청MDA함량,제고SOD활성,증강궤체청제자유기적능력；강저혈청IL-2함량,감소내부염증발생；종이제고공독궤체면역력,진이촉진공독적생장발육。
This article aimed to explore the effects of umbilical cord mesenchymal stem cells ( UC-MSCs ) transplantation on body weight ( BW) , serum immune and antioxidant levels of Holstein bull calves. Twenty eight 1-month-old Holstein bull calves with similar body weight were selected and randomly divided into control and experimental groups with 14 calves per group. Fresh fetal umbilical cord tissue was collected from healthy new born Holstein calves. UC-MSCs were purified and cultivated in vitro by the method of pancreatic enzyme digestion. Calves of experimental group were injected in jugular vein with 3×105 cells/kg BW UC-MSCs (dilu-ted to 8 mL) , while those of control group were injected with normal saline at equal dose. The injection was carried out at 1, 2, 3, 4 and 5 months of age, respectively. The test lasted for 150 d. The results showed as follows: compared with control group, the transplantation of UC-MSCs in bull calves, 1 ) significantly in-creased BW at 4, 5 and 6 months of age ( P<0. 05); significantly increased average daily gain at 3 ( P<0.05), 4 (P<0.05) and 6 months of age (P<0.01); 2) significantly increased serum immunoglobulin A ( IgA) content at 4 ( P<0.01) and 6 months of age ( P<0.05); significantly increased serum immunoglobulin G ( IgG) at 2 ( P<0.01) , 5 ( P<0.01) and 6 months of age ( P<0.05); significantly decreased serum inter-leukin-2 ( IL-2) content at 3 ( P<0.01) and 4 months of age ( P<0.05);3) significantly decreased serum ma-londialdehyde (MDA) content at 2, 3, 4, 5 and 6 months of age (P<0.01); significantly increased serum superoxide dismutase ( SOD) activity at 5 ( P<0.05) and 6 months of age ( P<0.01) . Thus, the transplanta-tion of UC-MSCs can increase serum IgA and IgG contents, decrease serum MDA content, increase serum SOD activity, enhance the body’ s ability to clear free radicals, reduced serum IL-2 content, and reduced inter-nal inflammation, thereby enhance immunity, and stimulate the growth and development of bull calves.