动物营养学报
動物營養學報
동물영양학보
ACTA ZOONUTRIMENTA SINICA
2015年
2期
503-509
,共7页
外源精胺%小肠%FGF2蛋白质表达量%形态结构%二糖酶%仔猪
外源精胺%小腸%FGF2蛋白質錶達量%形態結構%二糖酶%仔豬
외원정알%소장%FGF2단백질표체량%형태결구%이당매%자저
exogenous spermine%small intestine%FGF2 protein expression%morphology%disaccharidase%pig-lets
本试验旨在研究外源精胺对断奶仔猪小肠黏膜成纤维细胞因子-2( FGF2)蛋白质表达量及小肠发育的影响。将9窝胎次和初生体重相近的7日龄“杜×长×大”哺乳仔猪随机分配到0、12和15 mg/kg外源精胺组,每组3窝。仔猪从7日龄起补饲相应的饲粮,21日龄断奶后继续补饲断奶前饲粮至28日龄结束。从每窝选2头接近平均体重的仔猪屠宰,采集小肠及黏膜样品,测定FGF2蛋白质表达量和小肠的发育状况。结果表明：1)12 mg/kg外源精胺组仔猪小肠黏膜FGF2蛋白质表达量分别高于0和15 mg/kg外源精胺组,但3个外源精胺组仔猪小肠黏膜FGF2蛋白质表达量之间无显著差异(P>0.05)。2)12和15 mg/kg外源精胺组仔猪小肠绒毛高度和宽度分别极显著大于0 mg/kg外源精胺组( P<0.01),而12 mg/kg外源精胺组仔猪十二指肠和空肠黏膜绒毛高度与隐窝深度比值( V/C )分别极显著高于0 mg/kg外源精胺组( P<0.01)。3)28日龄仔猪小肠黏膜蔗糖酶和麦芽糖酶活性随外源精胺添加量的增加而升高。由此可见,给7~28日龄仔猪连续补饲外源精胺能提高28日龄仔猪小肠黏膜FGF2蛋白质表达量、V/C和二糖酶活性。
本試驗旨在研究外源精胺對斷奶仔豬小腸黏膜成纖維細胞因子-2( FGF2)蛋白質錶達量及小腸髮育的影響。將9窩胎次和初生體重相近的7日齡“杜×長×大”哺乳仔豬隨機分配到0、12和15 mg/kg外源精胺組,每組3窩。仔豬從7日齡起補飼相應的飼糧,21日齡斷奶後繼續補飼斷奶前飼糧至28日齡結束。從每窩選2頭接近平均體重的仔豬屠宰,採集小腸及黏膜樣品,測定FGF2蛋白質錶達量和小腸的髮育狀況。結果錶明：1)12 mg/kg外源精胺組仔豬小腸黏膜FGF2蛋白質錶達量分彆高于0和15 mg/kg外源精胺組,但3箇外源精胺組仔豬小腸黏膜FGF2蛋白質錶達量之間無顯著差異(P>0.05)。2)12和15 mg/kg外源精胺組仔豬小腸絨毛高度和寬度分彆極顯著大于0 mg/kg外源精胺組( P<0.01),而12 mg/kg外源精胺組仔豬十二指腸和空腸黏膜絨毛高度與隱窩深度比值( V/C )分彆極顯著高于0 mg/kg外源精胺組( P<0.01)。3)28日齡仔豬小腸黏膜蔗糖酶和麥芽糖酶活性隨外源精胺添加量的增加而升高。由此可見,給7~28日齡仔豬連續補飼外源精胺能提高28日齡仔豬小腸黏膜FGF2蛋白質錶達量、V/C和二糖酶活性。
본시험지재연구외원정알대단내자저소장점막성섬유세포인자-2( FGF2)단백질표체량급소장발육적영향。장9와태차화초생체중상근적7일령“두×장×대”포유자저수궤분배도0、12화15 mg/kg외원정알조,매조3와。자저종7일령기보사상응적사량,21일령단내후계속보사단내전사량지28일령결속。종매와선2두접근평균체중적자저도재,채집소장급점막양품,측정FGF2단백질표체량화소장적발육상황。결과표명：1)12 mg/kg외원정알조자저소장점막FGF2단백질표체량분별고우0화15 mg/kg외원정알조,단3개외원정알조자저소장점막FGF2단백질표체량지간무현저차이(P>0.05)。2)12화15 mg/kg외원정알조자저소장융모고도화관도분별겁현저대우0 mg/kg외원정알조( P<0.01),이12 mg/kg외원정알조자저십이지장화공장점막융모고도여은와심도비치( V/C )분별겁현저고우0 mg/kg외원정알조( P<0.01)。3)28일령자저소장점막자당매화맥아당매활성수외원정알첨가량적증가이승고。유차가견,급7~28일령자저련속보사외원정알능제고28일령자저소장점막FGF2단백질표체량、V/C화이당매활성。
This experiment was conducted to investigate the effects of exogenous spermine on small intestine mucous fibroblast growth factor-2 ( FGF2) protein expression and small intestinal development of weaner pig-lets. Nine litters of“Duroc×Landrace×Large White” crossbred piglets were randomly divided into three groups with 3 litters in each group, and piglets in the same group received a diet supplemented with exogenous sperm-ine at a level of 0, 12 and 15 mg/kg from 7 to 28 days of age. Two piglets with the average body weight from each litter were slaughtered and small intestinal samples were collected for the determination of FGF2 protein expression and small intestinal development. The results showed as follows: 1 ) the small intestine mucous FGF2 protein expression of piglets in 12 mg/kg exogenous spermine groups was higher than that in 0 and 15 mg/kg exogenous spermine groups, respectively. However, there was no significant difference on small intes-tine mucous FGF2 protein expression of piglets among 3 exogenous spermine groups (P>0.05). 2) The vil-lous height and width of piglets in 12 and 15 mg/kg exogenous spermine groups were significantly higher than that in 0 mg/kg exogenous spermine group ( P<0.01) , the villus height/crypt depth ( V/C) in duodenum and jejunum of piglets in 12 mg/kg exogenous spermine group were significantly higher than that in 0 mg/kg exog-enous spermine group ( P<0.01) , respectively. 3) The activities of sucrase and maltase in small intestine mu-cous of piglets at 28 days of age increased with the addition of exogenous spermine increased. In conclusion, dietary continuity supplemented with exogenous spermine of piglets at 7 to 28 days of age can increase small in-testine mucous FGF2 protein expression, V/C and disaccharidase activity of piglets at 28 days of age.
