CAJ | 학술논문

本试验旨在通过探讨单胃动物仿生消化系统测定鸭饲料酶水解物能值( EHGE)的可加性,进一步检验仿生消化方法的可行性。采用单因素完全随机设计,共12个处理,其中处理1~4分别为玉米、大豆粕、棉籽粕、小麦麸饲粮,处理5~12为以上2种或2种以上的饲料原料按不同比例组合制备的8种饲粮。每个处理5个重复,每个重复1根消化管。通过仿生消化系统测定各处理的干物质消化率( DMD)和EHGE,并根据饲粮中饲料原料的比例及其DMD和EHGE分别计算饲粮的DMD和EHGE。结果表明,8个饲粮DMD实测值的平均值显著高于计算值的平均值(分别为70.15%和69.48%,P=0.0009),但从DMD的计算值对实测值的线性回归看,模型的决定系数为0.9933( P<0.05),截距与0无显著性差异(截距=1.77,P=0.4683),斜率与1无显著性差异(截距=0.98,P=0.6474)。 DMD实测值的方差和变异系数分别显著低于计算值的方差和变异系数( P=0.0002和P=0.0010)。在计算值的方差对实测值方差的线性回归,计算值变异系数对实测值变异系数的线性回归上,模型的决定系数分别为0.0208( P>0.05)和0.1157( P>0.05)。8个饲粮EHGE实测值的平均值与计算值的平均值无显著性差异(分别为13.21和13.21 MJ/kg,P=0.9653)。从EHGE的计算值对实测值的线性回归看,模型的决定系数为0.9910( P<0.05),截距与0无显著性差异(截距=0.04,P=0.9419),斜率与1无显著性差异(截距=1.00,P=0.9400)。 EHGE实测值的方差平均值与计算值的方差平均值无显著差异( P=0.6811),而实测值变异系数的平均值与计算值的变异系数平均值有显著性差异( P=0.0036)。在计算值的方差对实测值方差的线性回归,计算值变异系数对实测值变异系数的线性回归上,模型的决定系数分别为0.2000(P>0.05)和0.0805(P>0.05)。综合上述结果,仿生消化法测定玉米、大豆粕、棉籽粕、小麦麸的DMD和EHGE在饲粮中具有可加性,然而测试数据的方差与变异系数不具有可加性。本試驗旨在通過探討單胃動物倣生消化繫統測定鴨飼料酶水解物能值( EHGE)的可加性,進一步檢驗倣生消化方法的可行性。採用單因素完全隨機設計,共12箇處理,其中處理1~4分彆為玉米、大豆粕、棉籽粕、小麥麩飼糧,處理5~12為以上2種或2種以上的飼料原料按不同比例組閤製備的8種飼糧。每箇處理5箇重複,每箇重複1根消化管。通過倣生消化繫統測定各處理的榦物質消化率( DMD)和EHGE,併根據飼糧中飼料原料的比例及其DMD和EHGE分彆計算飼糧的DMD和EHGE。結果錶明,8箇飼糧DMD實測值的平均值顯著高于計算值的平均值(分彆為70.15%和69.48%,P=0.0009),但從DMD的計算值對實測值的線性迴歸看,模型的決定繫數為0.9933( P<0.05),截距與0無顯著性差異(截距=1.77,P=0.4683),斜率與1無顯著性差異(截距=0.98,P=0.6474)。 DMD實測值的方差和變異繫數分彆顯著低于計算值的方差和變異繫數( P=0.0002和P=0.0010)。在計算值的方差對實測值方差的線性迴歸,計算值變異繫數對實測值變異繫數的線性迴歸上,模型的決定繫數分彆為0.0208( P>0.05)和0.1157( P>0.05)。8箇飼糧EHGE實測值的平均值與計算值的平均值無顯著性差異(分彆為13.21和13.21 MJ/kg,P=0.9653)。從EHGE的計算值對實測值的線性迴歸看,模型的決定繫數為0.9910( P<0.05),截距與0無顯著性差異(截距=0.04,P=0.9419),斜率與1無顯著性差異(截距=1.00,P=0.9400)。 EHGE實測值的方差平均值與計算值的方差平均值無顯著差異( P=0.6811),而實測值變異繫數的平均值與計算值的變異繫數平均值有顯著性差異( P=0.0036)。在計算值的方差對實測值方差的線性迴歸,計算值變異繫數對實測值變異繫數的線性迴歸上,模型的決定繫數分彆為0.2000(P>0.05)和0.0805(P>0.05)。綜閤上述結果,倣生消化法測定玉米、大豆粕、棉籽粕、小麥麩的DMD和EHGE在飼糧中具有可加性,然而測試數據的方差與變異繫數不具有可加性。
본시험지재통과탐토단위동물방생소화계통측정압사료매수해물능치( EHGE)적가가성,진일보검험방생소화방법적가행성。채용단인소완전수궤설계,공12개처리,기중처리1~4분별위옥미、대두박、면자박、소맥부사량,처리5~12위이상2충혹2충이상적사료원료안불동비례조합제비적8충사량。매개처리5개중복,매개중복1근소화관。통과방생소화계통측정각처리적간물질소화솔( DMD)화EHGE,병근거사량중사료원료적비례급기DMD화EHGE분별계산사량적DMD화EHGE。결과표명,8개사량DMD실측치적평균치현저고우계산치적평균치(분별위70.15%화69.48%,P=0.0009),단종DMD적계산치대실측치적선성회귀간,모형적결정계수위0.9933( P<0.05),절거여0무현저성차이(절거=1.77,P=0.4683),사솔여1무현저성차이(절거=0.98,P=0.6474)。 DMD실측치적방차화변이계수분별현저저우계산치적방차화변이계수( P=0.0002화P=0.0010)。재계산치적방차대실측치방차적선성회귀,계산치변이계수대실측치변이계수적선성회귀상,모형적결정계수분별위0.0208( P>0.05)화0.1157( P>0.05)。8개사량EHGE실측치적평균치여계산치적평균치무현저성차이(분별위13.21화13.21 MJ/kg,P=0.9653)。종EHGE적계산치대실측치적선성회귀간,모형적결정계수위0.9910( P<0.05),절거여0무현저성차이(절거=0.04,P=0.9419),사솔여1무현저성차이(절거=1.00,P=0.9400)。 EHGE실측치적방차평균치여계산치적방차평균치무현저차이( P=0.6811),이실측치변이계수적평균치여계산치적변이계수평균치유현저성차이( P=0.0036)。재계산치적방차대실측치방차적선성회귀,계산치변이계수대실측치변이계수적선성회귀상,모형적결정계수분별위0.2000(P>0.05)화0.0805(P>0.05)。종합상술결과,방생소화법측정옥미、대두박、면자박、소맥부적DMD화EHGE재사량중구유가가성,연이측시수거적방차여변이계수불구유가가성。
Additivity of enzyme hydrolysate gross energy ( EHGE ) of feedstuffs determined using computer-controlled simulated digestion system was investigated to validate the method for in vitro digestion. Twelve treatments consisted of 4 feedstuffs and 8 diets were used in a single factorial completely randomized arrange-ment. Treatments 1 to 4 were corn, soybean meal, cottonseed meal and wheat bran diets, respectively. Treat-ments 5 to 12 were 8 diets formulated with 2, 3 or 4 feedstuffs at different concentration. Each treatment con-tained 5 replicates with 1 digestion tube per replicate. The dry matter digestibility ( DMD) and EHGE of each treatment were determined using computer-controlled simulated digestion system. The calculated DMD and EH-GE of treatments 5 to 12 were estimated according to the determined DMD and EHGE of individual ingredients and their concentrations in the diets, respectively. The results showed as follows:the mean of determined DMD was greater than that of calculated values of 8 diets (70.15% and 69.48% for determined and calculated val-ues, respectively; P=0.000 9) . However, the regression of determined values on calculated values for DMD revealed that the coefficient of determination was 0.993 3 (P<0.05), and the intercept did not differ from 0 ( intercept=1.77, P=0.468 3) and slope did not differ from 1 ( slope=0.98, P=0.647 4) . The variance and coefficient of variation ( CV ) of determined values were less than those of calculated values for DMD ( P=0.000 2 and P=0.001 0 for variance and CV, respectively) . The regression of observed values on calculated values for variance and CV of DMD showed that the coefficients of determination were 0.020 8 ( P>0.05) and 0.115 7 (P>0.05), respectively. No significant difference was observed between the determined values and calculated values for EHGE (13.21 and 13.21 MJ/kg for determined and calculated values, respectively; P=0.965 3) . The regression of determined values on calculated values for EHGE revealed that the coefficient of determination was 0.991 0 ( P<0.05) , and the intercept did not differ from 0 ( intercept=0.04, P=0.941 9) and slope did not differ from 1 ( slope=1.00, P=0.940 0) . The differences between observed values and cal-culated values were not significant for variance (P=0.681 1), however, significant for CV (P=0.003 6). The regression of observed values on calculated values for variance and CV of DMD showed that the coeffi-cients of determination were 0.020 0 ( P>0.05) and 0.080 5 ( P>0.05) , respectively. In conclusion, the val-ues in corn, soybean meal, cottonseed meal and wheat bran are additive for DMD and EHGE in a complete di-et, respectively. However, the values for variance and CV are not additive, respectively.