CAJ | 학술논문

本试验旨在研究饲粮不同铜源及水平对育成期水貂血清铜、锌和铁含量及血清氮代谢、脂类和抗氧化指标影响,以及水貂对不同铜源的相对生物学利用率。选择60日龄健康、体重接近的水貂240只,随机分成10组,每组24个重复,每个重复1只貂。试验采用3×3+1试验设计,对照组饲喂基础饲粮,试验组分别在基础饲粮中添加3种铜源(硫酸铜、碱式氯化铜、蛋氨酸铜)和3个铜添加水平(10、25、40 mg/kg),预试期7 d,正试期45 d。结果表明：水貂血清铜含量随饲粮铜添加水平增加呈极显著线性增加( P=0.0004)。水貂血清总胆固醇( P=0.0015)、甘油三酯( P=0.0021)和高密度脂蛋白胆固醇( P=0.0101)含量随饲粮铜添加水平增加呈显著或极显著线性降低,然而水貂血清低密度脂蛋白胆固醇含量却未受饲粮铜添加水平的影响(P=0.5790)。水貂血清铜蓝蛋白和铜锌超氧化物歧化酶活性均随饲粮铜添加水平增加呈极显著线性升高( P=0.0001)。以血清铜蓝蛋白活性为指标,以硫酸铜为参比标准物(100%),碱式氯化铜和蛋氨酸铜的相对生物学利用率分别为109.93%和131.06%。以血清铜锌超氧化物歧化酶活性为指标,以硫酸铜为参比标准物(100%),碱式氯化铜和蛋氨酸铜的相对生物学利用率分别为111.01%和118.10%。由此可见,饲粮添加40 mg/kg的铜(基础饲粮铜含量8.05 mg/kg)能提高育成期水貂血清抗氧化酶的活性；育成期水貂3组铜源的相对生物学利用率为：蛋氨酸螯合铜>碱式氯化铜>硫酸铜。
본시험지재연구사량불동동원급수평대육성기수초혈청동、자화철함량급혈청담대사、지류화항양화지표영향,이급수초대불동동원적상대생물학이용솔。선택60일령건강、체중접근적수초240지,수궤분성10조,매조24개중복,매개중복1지초。시험채용3×3+1시험설계,대조조사위기출사량,시험조분별재기출사량중첨가3충동원(류산동、감식록화동、단안산동)화3개동첨가수평(10、25、40 mg/kg),예시기7 d,정시기45 d。결과표명：수초혈청동함량수사량동첨가수평증가정겁현저선성증가( P=0.0004)。수초혈청총담고순( P=0.0015)、감유삼지( P=0.0021)화고밀도지단백담고순( P=0.0101)함량수사량동첨가수평증가정현저혹겁현저선성강저,연이수초혈청저밀도지단백담고순함량각미수사량동첨가수평적영향(P=0.5790)。수초혈청동람단백화동자초양화물기화매활성균수사량동첨가수평증가정겁현저선성승고( P=0.0001)。이혈청동람단백활성위지표,이류산동위삼비표준물(100%),감식록화동화단안산동적상대생물학이용솔분별위109.93%화131.06%。이혈청동자초양화물기화매활성위지표,이류산동위삼비표준물(100%),감식록화동화단안산동적상대생물학이용솔분별위111.01%화118.10%。유차가견,사량첨가40 mg/kg적동(기출사량동함량8.05 mg/kg)능제고육성기수초혈청항양화매적활성；육성기수초3조동원적상대생물학이용솔위：단안산오합동>감식록화동>류산동。
This experiment was conducted to study the effects of different copper sources and levels on serum copper, zinc and iron contents, nitrogen metabolism, lipid and antioxidant indices of minks in late growing pe-riod, and different copper sources relative bioavailability of minks. Two hundred and forty 60-day-old minks were randomly divided into 10 groups with 24 replicates per group and 1 mink per replicate. The experiment was used in a 3×3+1 factorial experiment design, minks in the control group were fed a basal diet, and minks in other nine groups were fed basal diets supplemented with 3 sources ( copper sulfate, tribasic copper chloride and copper methionine) and 3 levels (10, 25, and 40 mg/kg). The pre-test period lasted for 7 days, and the trial lasted for 45 days. The results showed as follows:the serum copper content of minks was significant linear increased with dietary copper supplemental level increased ( P=0.000 4) , the contents of total cholesterol ( P=0.001 5), triglyceride (P=0.002 1) and high density lipoprotein cholesterol (P=0.010 1) in serum of minks were significant linear decreased with dietary copper supplemental level increased, but dietary copper supple-mental level had no significant effect on serum high density lipoprotein cholesterol content of minks ( P=0.579 0). The activities of ceruloplasmin (CRE) and copper zinc superoxide dismutase (Cu-Zn SOD) in ser-um of minks were significant linear increased with dietary copper supplemental level increased ( P=0.000 1) . Compared with copper sulfate ( 100%) , the relative bioavailability of tribasic copper chloride and copper me-thionine were 109.93% and 131.06% based on serum CER activity, respectively. Compared with copper sul-fate (100%), the relative bioavailability of tribasic copper chloride and copper methionine were 111.01% and 118.10% based on serum Cu-Zn SOD activity, respectively. The results indicate that dietary supplemented with 40 mg/kg copper ( copper content in basal diet is 8.05 mg/kg) can improve serum antioxidant enzyme activity of minks in late growing period, the relative bioavailability of 3 copper sources is copper methionine>tribasic copper chloride>copper sulfate.