国际眼科杂志
國際眼科雜誌
국제안과잡지
INTERNATIONAL JOURNAL OF OPHTHALMOLOGY
2015年
2期
198-200
,共3页
过氧化物酶增殖激活受体-y共激活子-1α%血管内皮生长因子%重组蛋白质类%常氧%低氧
過氧化物酶增殖激活受體-y共激活子-1α%血管內皮生長因子%重組蛋白質類%常氧%低氧
과양화물매증식격활수체-y공격활자-1α%혈관내피생장인자%중조단백질류%상양%저양
peroxisome - proliferator - activated receptor-γ coactivator- 1α%vascular endothelial growth factor%recombinant proteins%normoxia%hypoxia
目的：：探讨外源性过氧化物酶增殖激活受体-y共激活子-1α( peroxisome-proliferator-activated receptor-y coactivator-1α, PGC-1α)对人视网膜血管内皮细胞( human retinal vascular endothelial cell, HRVEC )中血管内皮生长因子( vascular endothelial growth factor,VEGF)表达的影响。方法：HRVEC加入重组PGC-1α蛋白,未加入重组蛋白的细胞作为对照组。加入重组蛋白24 h后,将两组细胞分别置于常氧(20% O2)和低氧(1% O2)环境下继续培养16h,采用real-time PCR、酶联免疫吸附试验( ELISA)及免疫荧光细胞化学法分别检测VEGF mRNA和蛋白的表达变化。结果：real-time PCR,ELISA和免疫荧光细胞化学法检测发现,常氧和低氧环境下HRVEC加入重组PGC-1α蛋白后,VEGF mRNA和蛋白的表达均较对照组上调,差异有统计学意义(P<0.01)。结论：PGC-1α在常氧和低氧环境中均能上调HRVEC中VEGF的表达。
目的：：探討外源性過氧化物酶增殖激活受體-y共激活子-1α( peroxisome-proliferator-activated receptor-y coactivator-1α, PGC-1α)對人視網膜血管內皮細胞( human retinal vascular endothelial cell, HRVEC )中血管內皮生長因子( vascular endothelial growth factor,VEGF)錶達的影響。方法：HRVEC加入重組PGC-1α蛋白,未加入重組蛋白的細胞作為對照組。加入重組蛋白24 h後,將兩組細胞分彆置于常氧(20% O2)和低氧(1% O2)環境下繼續培養16h,採用real-time PCR、酶聯免疫吸附試驗( ELISA)及免疫熒光細胞化學法分彆檢測VEGF mRNA和蛋白的錶達變化。結果：real-time PCR,ELISA和免疫熒光細胞化學法檢測髮現,常氧和低氧環境下HRVEC加入重組PGC-1α蛋白後,VEGF mRNA和蛋白的錶達均較對照組上調,差異有統計學意義(P<0.01)。結論：PGC-1α在常氧和低氧環境中均能上調HRVEC中VEGF的錶達。
목적：：탐토외원성과양화물매증식격활수체-y공격활자-1α( peroxisome-proliferator-activated receptor-y coactivator-1α, PGC-1α)대인시망막혈관내피세포( human retinal vascular endothelial cell, HRVEC )중혈관내피생장인자( vascular endothelial growth factor,VEGF)표체적영향。방법：HRVEC가입중조PGC-1α단백,미가입중조단백적세포작위대조조。가입중조단백24 h후,장량조세포분별치우상양(20% O2)화저양(1% O2)배경하계속배양16h,채용real-time PCR、매련면역흡부시험( ELISA)급면역형광세포화학법분별검측VEGF mRNA화단백적표체변화。결과：real-time PCR,ELISA화면역형광세포화학법검측발현,상양화저양배경하HRVEC가입중조PGC-1α단백후,VEGF mRNA화단백적표체균교대조조상조,차이유통계학의의(P<0.01)。결론：PGC-1α재상양화저양배경중균능상조HRVEC중VEGF적표체。
AlM: To investigate the effect of exogenous peroxisome-proliferator-activated receptor-γcoactivator-1α ( PGC-1α) on vascular endothelial growth factor ( VEGF) expression in human retinal vascular endothelial cells ( HRVEC) .METHODS:Recombinant PGC-1α protein was added to HRVEC, and no recombinant PGC-1α protein was added to HRVEC as control group. After 24h of incubation, two groups of cells were then placed into a normoxic ( 20%O2 ) or hypoxic ( 1% O2 ) environment for another 16h. The expression of VEGF mRNA and protein were detected by real - time PCR, ELlSA and immunofluorescence cytochemistry.RESULTS: VEGF mRNA and protein levels in the cells were significantly increased by recombinant PGC - 1αprotein both under normoxia and hypoxia conditions as compared with control groups (P<0. 01).CONCLUSlON: PGC-1α can upregulate the expression of VEGF in HRVEC under normoxia and hypoxia conditions.
