中国临床药理学杂志
中國臨床藥理學雜誌
중국림상약이학잡지
THE CHINESE JOURNAL OF CLINICAL PHARMACOLOGY
2015年
2期
119-121
,共3页
苯妥英钠%苯巴比妥%卡马西平%超高效液相色谱法%血清
苯妥英鈉%苯巴比妥%卡馬西平%超高效液相色譜法%血清
분타영납%분파비타%잡마서평%초고효액상색보법%혈청
phenytoin sodium%phenobarbital%carbamazepine%ultra performance liquid chromatography%serum
目的：建立超高效液相色谱（ UPLC）法测定血清中苯妥英钠、苯巴比妥和卡马西平的浓度。方法血清样品经乙腈沉淀蛋白后进样分析。色谱柱为ACQUITY UPLC BEH C18色谱柱，流动相为乙腈－水（28∶72），流速：0.3 mL· min－1，检测波长220 nm，柱温20℃。结果苯妥英钠、苯巴比妥和卡马西平的血清浓度分别在2.5～80.0，5.0～160.0，1.0～32.0μg· mL－1线性关系良好，日内、日间精密度（ RSD）均小于7.28％，提取回收率为96.18％～103.89％。结论本方法简便、快速、准确，可用于测定血清中苯妥英钠、苯巴比妥和卡马西平的浓度。
目的：建立超高效液相色譜（ UPLC）法測定血清中苯妥英鈉、苯巴比妥和卡馬西平的濃度。方法血清樣品經乙腈沉澱蛋白後進樣分析。色譜柱為ACQUITY UPLC BEH C18色譜柱，流動相為乙腈－水（28∶72），流速：0.3 mL· min－1，檢測波長220 nm，柱溫20℃。結果苯妥英鈉、苯巴比妥和卡馬西平的血清濃度分彆在2.5～80.0，5.0～160.0，1.0～32.0μg· mL－1線性關繫良好，日內、日間精密度（ RSD）均小于7.28％，提取迴收率為96.18％～103.89％。結論本方法簡便、快速、準確，可用于測定血清中苯妥英鈉、苯巴比妥和卡馬西平的濃度。
목적：건립초고효액상색보（ UPLC）법측정혈청중분타영납、분파비타화잡마서평적농도。방법혈청양품경을정침정단백후진양분석。색보주위ACQUITY UPLC BEH C18색보주，류동상위을정－수（28∶72），류속：0.3 mL· min－1，검측파장220 nm，주온20℃。결과분타영납、분파비타화잡마서평적혈청농도분별재2.5～80.0，5.0～160.0，1.0～32.0μg· mL－1선성관계량호，일내、일간정밀도（ RSD）균소우7.28％，제취회수솔위96.18％～103.89％。결론본방법간편、쾌속、준학，가용우측정혈청중분타영납、분파비타화잡마서평적농도。
Objective To establish an ultra performance liquid chroma-tography ( UPLC) method for the determination of phenytoin sodium , phe-nobarbital and carbamazepine in human serum.Methods Serum sam-ples were injected and analyzed after protein precipitation with acetoni-trile.The analysis was performed on an ACQUITY UPLC BEH C 18 column, and the mobile phase consisted of acetonitrile -water (28∶72), the flow rate was 0.3 mL · min-1 , the detection wavelength was set at 220 nm and the temperature was maintained at 20 ℃.Results The peak area and content of phenytoin sodium , phenobarbital and carbama-zepine showed good linearity in the range of 2.5-80.0, 5.0-160.0 and 1.0-32.0 μg · mL-1 , respectively.Intra-and inter -day RSDs were all lower than 7.28%. The recoveries of phenytoin sodium were 96.18%-103.89%.Conclusion The established method is simple , accurate and rapid , and can be applied to determination of phenytoin sodium , phenobarbital and carbamazepine in human serum.
