CAJ | 학술논문

目的：建立表面解吸常压化学电离质谱（ SDAPCI－MS）技术检测鲍曼不动杆菌特有的挥发性代谢产物（VCs）以便快速鉴定鲍曼不动杆菌。方法复苏临床分离获得的鲍曼不动杆菌10株，接种到血琼脂平板上，37℃孵育培养，用SDAPCI－MS对培养不同时间的鲍曼不动杆菌进行VCs检测。结果与空白培养基比较，检测到鲍曼不动杆菌特有的VCs及特征的质荷比峰（ m／z 60和118），且鲍曼不动杆菌在不同培养时间产生的VCs基本相同。结论 SDAPCI－MS有望通过特征性VCs对鲍曼不动杆菌进行快速检测。
목적：건립표면해흡상압화학전리질보（ SDAPCI－MS）기술검측포만불동간균특유적휘발성대사산물（VCs）이편쾌속감정포만불동간균。방법복소림상분리획득적포만불동간균10주，접충도혈경지평판상，37℃부육배양，용SDAPCI－MS대배양불동시간적포만불동간균진행VCs검측。결과여공백배양기비교，검측도포만불동간균특유적VCs급특정적질하비봉（ m／z 60화118），차포만불동간균재불동배양시간산생적VCs기본상동。결론 SDAPCI－MS유망통과특정성VCs대포만불동간균진행쾌속검측。
Objective To establish the rapid identification of Acineto-bacter baumannii by detecting specific volatile metabolites of Acinetobacter baumannii through surface desorption atmospheric pressure chemical ioni-zation mass spectrometry ( SDAPCI-MS).Methods Ten clinical isola-ted Acinetobacter baumannii were resuscitated and vaccinated to blood agar plates at 37 ℃incubation and culturing.SDAPCI-MS was adopted to detect volatile metabolites of Acinetobacter baumannii by different cul-turing time.Results Compared with blank culture medium , the pecu-liar volatile metabolites were detected ,the characteristic peaks are mass -to-charge ratio 60 and 118.Basically the same volatile metabolites of Acinetobacter baumannii were generated by different culturing time.Con-clusion The rapid identification of Acinetobacter baumannii through peculiar volatile metabolites is hopefully applied by SDAPCI -MS.