CAJ | 학술논문

目的：：探讨脂肪细胞因子 Chemerin在冠心病( CHD)发生与发展中的临床价值。方法：将20只健康雄性apoE-/-小鼠随机分为普食喂养组(NF组)10只和高脂喂养组(HF组)10只,实时荧光定量PCR(QPCR)检测2组小鼠腹主动脉旁脂肪中Chemerin mRNA相对表达水平；ELISA方法检测2组小鼠血清中Chemerin的含量；采用ELISA方法检测45例正常人及150例疑似CHD患者血清中Chemerin,其中疑似CHD患者经临床相关检测确诊为心绞痛25例,心肌梗死115例,心肌损伤非冠心病10例。结果：(1)小鼠腹主动脉旁脂肪组织中Chemerin mRNA相对表达水平HF组较NF组明显升高(P<0．01)。(2)小鼠血清中Chemerin含量HF组较NF组也明显升高(P<0．05)。(3)人血清中Chemerin含量冠心病患者(最终确诊为心绞痛及心肌梗死)较正常人群明显升高(P<0．05),其中心肌梗死患者较心绞痛患者Chemerin水平明显增加(P<0．05)。结论：在小鼠动脉粥样硬化斑块形成过程中Chemerin水平的升高提示其可能参与了促动脉粥样硬化斑块的形成。在正常人及冠心病患者尤其是心肌梗死患者的血清中Chemerin水平的改变表明其可能参与了冠心病尤其是心肌梗死的发生与发展,具有一定的预警作用。
목적：：탐토지방세포인자 Chemerin재관심병( CHD)발생여발전중적림상개치。방법：장20지건강웅성apoE-/-소서수궤분위보식위양조(NF조)10지화고지위양조(HF조)10지,실시형광정량PCR(QPCR)검측2조소서복주동맥방지방중Chemerin mRNA상대표체수평；ELISA방법검측2조소서혈청중Chemerin적함량；채용ELISA방법검측45례정상인급150례의사CHD환자혈청중Chemerin,기중의사CHD환자경림상상관검측학진위심교통25례,심기경사115례,심기손상비관심병10례。결과：(1)소서복주동맥방지방조직중Chemerin mRNA상대표체수평HF조교NF조명현승고(P<0．01)。(2)소서혈청중Chemerin함량HF조교NF조야명현승고(P<0．05)。(3)인혈청중Chemerin함량관심병환자(최종학진위심교통급심기경사)교정상인군명현승고(P<0．05),기중심기경사환자교심교통환자Chemerin수평명현증가(P<0．05)。결론：재소서동맥죽양경화반괴형성과정중Chemerin수평적승고제시기가능삼여료촉동맥죽양경화반괴적형성。재정상인급관심병환자우기시심기경사환자적혈청중Chemerin수평적개변표명기가능삼여료관심병우기시심기경사적발생여발전,구유일정적예경작용。
Objective:To explore the clinical value of Chemerin in the occurrence and development of coronary heart disease (CHD). Methods:Healthy male ApoE-/ - mice were randomly divided into normal-chow group (NF group, n=10) and high-fat fed group (HF group, n=10). THE mRNA expression of Chemerin were measured by QPCR in mice hepatic tissue. The level of serum Chemerin were determined by ELISA. The levels of serum Chemerin in 45 cases of normal control group and in 150 cases of CHD suspected group were measured with ELISA. Results:( 1 ) Chemerin mRNA relative expression levels of mice in abdominal aorta adipose tissue:in HF group was compared with in NF group, there was significantly difference (P<0. 01). (2) The serum Chemerin levels of mice:in HF group than the in NF group was significantly increased (P<0. 05) and Chemerin level of the serum were increased (P<0. 05). (3)The levels of serum Chemerin of the normal population and patients with CHD: in CHD group (eventually diagnosed as angina and myocardial infarction) was compared with in the normal population,there was significantly difference (P<0. 05), Chemerin levels of myocardial infarction was increased more than angina group(P<0. 05). Conclusion:Chemerin level increase in the process of formation of atherosclerotic plaques in mice may be involved in promoting atherosclerotic plaque formation. Changes of Chemerin level may be involved in the occurrence and development of CHD and MI, there is a role in early warning for CHD.