CAJ | 학술논문

本文提出一种基于抗生素作用下的微生物膜阻抗及代谢液微量残留物分析的电化学并行检测平台,从量化角度实时反映抗生素对微生物的膜贴附变化及代谢成分的影响。根据现有抗生素电化学检测单元的检测精度,待测生理溶液的进样体积需控制在20μL/min~100μL/min,因此本文针对平台中的关键部分———微泵单元采用有限元设计方法进行了静力场、动力场和流体场的综合分析,确保微泵工作流量的精确性和可控性。分析结果得到设计的微泵在施加20 Hz,±40 V驱动电压时的流量为52.864μL/min,并得出流量与电压及频率的关系式来控制流量的变化；在该基础上对微泵被动阀做了力学特性分析与优化设计,分析得出阀臂固支端优化尺寸为0.4 mm×0.5 mm；最后以商用PSS20型微泵为仿真对象做了相关验证性实验,得到仿真流量的误差率为6.7%,验证了本方法的可行性和准确性,为后期微泵的制作、改进以及多参数检测平台的搭建提供了良好的设计参考。
본문제출일충기우항생소작용하적미생물막조항급대사액미량잔류물분석적전화학병행검측평태,종양화각도실시반영항생소대미생물적막첩부변화급대사성분적영향。근거현유항생소전화학검측단원적검측정도,대측생리용액적진양체적수공제재20μL/min~100μL/min,인차본문침대평태중적관건부분———미빙단원채용유한원설계방법진행료정력장、동력장화류체장적종합분석,학보미빙공작류량적정학성화가공성。분석결과득도설계적미빙재시가20 Hz,±40 V구동전압시적류량위52.864μL/min,병득출류량여전압급빈솔적관계식래공제류량적변화；재해기출상대미빙피동벌주료역학특성분석여우화설계,분석득출벌비고지단우화척촌위0.4 mm×0.5 mm；최후이상용PSS20형미빙위방진대상주료상관험증성실험,득도방진류량적오차솔위6.7%,험증료본방법적가행성화준학성,위후기미빙적제작、개진이급다삼수검측평태적탑건제공료량호적설계삼고。
There are some crucial problems in design of parallel multi-parameter detection platform. This paper puts forward a parallel detection platform by electrochemistry method to analyze the antibiotic residue components in micro-bial metabolites,which quantitatively showed the influence of antibiotics on physiological environment,membrane ad-herence and metabolites of microorganism in real time. According to the required accuracy of antibiotic detection lim-it,the fluid volume for single detection should be controlled within 20 μL/min~100 μL/min. Therefore,this paper comprehensively analyzed the static field,dynamic field and fluid field of the micro pump by the finite element method to make sure that the precision and controllability of pump design meet the measurement requirements,which was the key point in the detection platform. Results showed that the micro-pump flow rate was 52.864μL/min when the pump was driven by 20 Hz,±40 V AC voltage,and the relations between frequency and voltage to control the real-time change of flow rate were also discussed. Moreover,the passive valve micro pump was further analyzed on mechanical characteristics and the optimized pump fix end size(0.4 mm×0.5 mm). Finally,verified experiment of the obtained simulation data for flow rate compared with PSS20 commercial micro-pump was done with the error rate of 6. 7%, which proved the feasibility and accuracy of the finite element method,and provided a good reference for the design and improvement of micro-pump as well as the parallel multi-parameter detection platform.