CAJ | 학술논문

制备了二氧化硅包裹的具有“金属-有机物-金属”三明治结构的表面增强拉曼散射( SERS)光学免疫检测探针，研究了探针的形貌、结构、组分等多种因素对其SERS特性的影响，探索出具有较好SERS活性的制备参数。之后利用探针上集成的SERS信号，采用三明治免疫法，实现了激酶抑制因子( p21)和人体抑癌基因( p53)两种疾病因子的高特异性检测。
제비료이양화규포과적구유“금속-유궤물-금속”삼명치결구적표면증강랍만산사( SERS)광학면역검측탐침，연구료탐침적형모、결구、조분등다충인소대기SERS특성적영향，탐색출구유교호SERS활성적제비삼수。지후이용탐침상집성적SERS신호，채용삼명치면역법，실현료격매억제인자( p21)화인체억암기인( p53)량충질병인자적고특이성검측。
A novel kind of silica coated‘metal-organic-metal ’ sandwich-type surface enhanced Raman scattering (SERS)nanoprobe has been successfully prepared. To explore the optimal preparation condition,the influences of morphology,structure and composition on the SERS performance of the nanoprobe were carefully investigated. After successful fabrication of the nanoprobe,its application in the detection of two biomarkers( p21 and p53) was investi-gated by using a sandwich-type immunoassay protocol. The results confirmed that the nanoprobe can generate strong SERS signals and can distinguish p21 and p53 with a good specificity.