CAJ | 학술논문

根据己公布的神经坏死病毒全基因序列，应用引物设计软件依据GeneBank所收录的各种石斑鱼神经坏死病毒RNA2基因组全序列选择保守区域，根据Taqman探针引物设计原则，设计TaqMan探针及其引物。采用TaqMan探针技术，并且将假病毒技术构建的含有神经坏死病毒RNA2基因组的MS2噬菌体假病毒作为阳性质控品，建立TaqMan探针检测点带石斑鱼神经坏死病毒的荧光定量PCR方法，拟合标准曲线，能够在病毒检测中进行绝对定量，能检测到10个病毒拷贝模板数，在临床检测中得到很好运用。本研究建立实时荧光定量RT-PCR，有助于日常检验工作，为鱼类神经坏死病毒的筛选和监测提供基础，有助于控制神经坏死病的疫情传播。
근거기공포적신경배사병독전기인서렬，응용인물설계연건의거GeneBank소수록적각충석반어신경배사병독RNA2기인조전서렬선택보수구역，근거Taqman탐침인물설계원칙，설계TaqMan탐침급기인물。채용TaqMan탐침기술，병차장가병독기술구건적함유신경배사병독RNA2기인조적MS2서균체가병독작위양성질공품，건립TaqMan탐침검측점대석반어신경배사병독적형광정량PCR방법，의합표준곡선，능구재병독검측중진행절대정량，능검측도10개병독고패모판수，재림상검측중득도흔호운용。본연구건립실시형광정량RT-PCR，유조우일상검험공작，위어류신경배사병독적사선화감측제공기출，유조우공제신경배사병적역정전파。
According to the study published by the others nervous necrosis virus gene sequence, application primer design software based on GeneBank contains various grouper nervous necrosis virus RNA genome sequence choose conservative region while TaqMan probe design and primers. TaqMan probe technology, and will leave Construction of the virus containing nerve necrosis virus RNA genome of the virus MS2 bacteriophage as a positive controls, a TaqMan probe brings grouper nervous necrosis virus fluorescence quantitative PCR, and the establishment of a standard curve, which can detect virus testing for absolute quantification, the minimum can detect 10 viruses, In actual use of detection achieved very good results. The establishment of real-time quantitative RT-PCR and contribute to future coastal ports in the day-to-day inspection and fish and nervous necrosis virus monitoring provided by the founda-tion, will help to control the nervous necrosis disease spread of the epidemic.