CAJ | 학술논문

目的：筛选优化沙棘粉中儿茶素的提取方法，并用HPLC测定其含量。方法：以均匀设计法优化提取条件，采用Agilent ZORBAX SB-C18柱，以甲醇—乙酸溶液（用乙酸调pH值至3.2）（25：75）为流动相，流速1 mL/min；柱温25℃，检测波长280nm。结果：优选出的最佳提取条件：甲醇浓度为74.0%，水浴温度为70℃，超声时间为30min。儿茶素在0.50～10.0μg/mL 浓度范围内线性良好，相关系数（r）为0.9999，平均回收率为97.25%（RSD=1.77%）。结论：本试验建立的含量测定方法操作简便、重现性好且具有较强的专属性。
목적：사선우화사극분중인다소적제취방법，병용HPLC측정기함량。방법：이균균설계법우화제취조건，채용Agilent ZORBAX SB-C18주，이갑순—을산용액（용을산조pH치지3.2）（25：75）위류동상，류속1 mL/min；주온25℃，검측파장280nm。결과：우선출적최가제취조건：갑순농도위74.0%，수욕온도위70℃，초성시간위30min。인다소재0.50～10.0μg/mL 농도범위내선성량호，상관계수（r）위0.9999，평균회수솔위97.25%（RSD=1.77%）。결론：본시험건립적함량측정방법조작간편、중현성호차구유교강적전속성。
Objective:Screening methods to optimize the extraction of catechins sea buckthorn powder,and determine its content by HPLC. Methods:With uniform design to optimize ultrasonic extraction conditions,using Agilent ZORBAX SB-C18 column with methanol - acetic acid solution(pH value adjusted to 3.2 with acetic acid)(25:75)as the mobile phase, flow rate 1 mL / min;column temperature was 25 ℃ The detection wavelength 280nm. Results:The optimum extraction con?ditions optimized concentration of 74.0% for methanol,water bath temperature of 70 ℃,ultrasonic time of 30min. Catechin at 0.50 ~ 10.0μg / mL concentration range good linearity,the correlation coefficient(r)was 0.9999,the average recovery was 97.25%(RSD=1.77%). Conclusion:Method for the determination of the trial established simple,reproducible and has a strong specificity.