福建分析测试
福建分析測試
복건분석측시
FUJIAN ANALYSIS & TESTING
2015年
1期
36-38
,共3页
丹参饮片%丹参酮ⅡA%高效液相色谱法
丹參飲片%丹參酮ⅡA%高效液相色譜法
단삼음편%단삼동ⅡA%고효액상색보법
Radix salviae miltiorrhizae%Tanshinone ⅡA%HPLC
建立了高效液相色谱测定丹参饮片中丹参酮ⅡA 含量的方法。样品以乙醇为溶剂进行提取，提取液在Eclipse Plus C18柱色谱柱上，以70％的乙腈水溶液为流动相进行分离，检测波长为268nm。丹参酮 IIA 在1.6×10-4-0.05g/L范围内呈现良好线性关系（r=0.999），检测限为4.7×10-5 g/L。该方法具有操作简单、灵敏度高、重现性好等特点，应用方法准确测定了丹参饮片中丹参酮ⅡA的含量。
建立瞭高效液相色譜測定丹參飲片中丹參酮ⅡA 含量的方法。樣品以乙醇為溶劑進行提取，提取液在Eclipse Plus C18柱色譜柱上，以70％的乙腈水溶液為流動相進行分離，檢測波長為268nm。丹參酮 IIA 在1.6×10-4-0.05g/L範圍內呈現良好線性關繫（r=0.999），檢測限為4.7×10-5 g/L。該方法具有操作簡單、靈敏度高、重現性好等特點，應用方法準確測定瞭丹參飲片中丹參酮ⅡA的含量。
건립료고효액상색보측정단삼음편중단삼동ⅡA 함량적방법。양품이을순위용제진행제취，제취액재Eclipse Plus C18주색보주상，이70％적을정수용액위류동상진행분리，검측파장위268nm。단삼동 IIA 재1.6×10-4-0.05g/L범위내정현량호선성관계（r=0.999），검측한위4.7×10-5 g/L。해방법구유조작간단、령민도고、중현성호등특점，응용방법준학측정료단삼음편중단삼동ⅡA적함량。
A method for determination of tanshinoneⅡA in radix salviae miltiorrhizae by High performance liquid chroma?tography was developed. Sample was extracted by ethanol,and the extract was separated on an Eclipse Plus C18 column with 70% acetonitrile water solution as the mobile phase,The detection wavelength was set at 268nm. The calibration curve of tanshinoneⅡA showed good linearity in the range of 1.6×10-4-0.05g/L,then correlation coefficients R=0.999. The meth?od has advantages of simplicity,high sensitivity and good reproducibility,it can be used for the determination of tanshinoneⅡA in radix salviae miltiorrhizae.
