CAJ | 학술논문

采用平板涂布法利用3种培养基从青岛即墨市田横镇泊子盐场盐池底泥样中共分离纯化出15株放线菌，对分离菌株进行16S rRNA基因测序分析。发现这15株放线菌分属于链霉菌属（ Streptomyces ）、拟诺卡氏菌属（Nocardiopsis）和1个新属，其中 Streptomyces属为优势菌属；菌株CXB832与 Nocardiopsis arabia DSM 45083T和 Haloacti-nospora albus DSM 45015T最接近，同源性分别为95．4％和94．9％，根据其表型和分子特征可以初步判定该菌株为放线菌新属。培养基及培养温度对盐池环境中放线菌的分离效果均有影响，利用淀粉酪素培养基在37℃分离的放线菌种类和数量较多。在15株放线菌中，分别有12株、3株、2株和1株放线菌产淀粉酶、酯酶、蛋白酶和纤维素酶。
채용평판도포법이용3충배양기종청도즉묵시전횡진박자염장염지저니양중공분리순화출15주방선균，대분리균주진행16S rRNA기인측서분석。발현저15주방선균분속우련매균속（ Streptomyces ）、의낙잡씨균속（Nocardiopsis）화1개신속，기중 Streptomyces속위우세균속；균주CXB832여 Nocardiopsis arabia DSM 45083T화 Haloacti-nospora albus DSM 45015T최접근，동원성분별위95．4％화94．9％，근거기표형화분자특정가이초보판정해균주위방선균신속。배양기급배양온도대염지배경중방선균적분리효과균유영향，이용정분락소배양기재37℃분리적방선균충류화수량교다。재15주방선균중，분별유12주、3주、2주화1주방선균산정분매、지매、단백매화섬유소매。
In this study ,15 actinomycetes were isolated using three different media (M1~M3 ) from a salt pond of Qingdao .Bioinformatics analysis of the 16S rRNA sequences from the isolates revealed they belonged to three genera (Streptomyces ,Nocardiopsis and a novel genus) ,with Streptomyces being the dominant genus .The strain CXB832 were 95 .4 % and 94 .9 % homologous to the two standard strains of bacteria ,and therefore was candidates for a new genus on the basis of phenotypic ,chemotaxonomic and phylogenetic distinctiveness .The type of medium and temperature showed significant effects on the number and diversity of actinomycetes recovered .Starch casein medium was the most effective media to isolate actinomycetes species at 37℃ .12 ,3 ,2 and 1 of 15 isolates produces amylase ,lipase ,caseinase and cellulose ,respectively .