CAJ | 학술논문

尾砂中含有大量的金属，是一种潜在的可利用资源。旨在研发一种从铜尾砂中回收铜的生物浸出方法。利用正交设计，通过摇瓶试验，研究培养温度、发酵时间、接种量及培养基成分对黑曲霉菌丝球形态的影响，进而研究不同菌丝球形态的黑曲霉发酵液对尾砂中铜的浸出。研究表明，铜的浸出率随着黑曲霉菌丝球直径的减小而升高。在温度30℃，接种量0.8%（OD600=0.1），马铃薯-蔗糖含量30%，发酵时间65 h的优化发酵条件下，获得大量表面光滑、直径为0.96 mm的菌丝球；利用该条件下的发酵液，在30℃，180 r·min?1条件下浸出3 d，铜的浸出率达83.25%。
미사중함유대량적금속，시일충잠재적가이용자원。지재연발일충종동미사중회수동적생물침출방법。이용정교설계，통과요병시험，연구배양온도、발효시간、접충량급배양기성분대흑곡매균사구형태적영향，진이연구불동균사구형태적흑곡매발효액대미사중동적침출。연구표명，동적침출솔수착흑곡매균사구직경적감소이승고。재온도30℃，접충량0.8%（OD600=0.1），마령서-자당함량30%，발효시간65 h적우화발효조건하，획득대량표면광활、직경위0.96 mm적균사구；이용해조건하적발효액，재30℃，180 r·min?1조건하침출3 d，동적침출솔체83.25%。
Owing to the substantial amounts of metals embodied in tailings, they are potential viable sources of metals. This work aims at developing a technique to microbially recover copper present in tailings. Based on orthogonal design, the effects of different variables, such as temperature, fermentation time, inoculum size and medium components, on pellet morphology of A. niger were investigated in shake flasks. Leaching of copper from its tailings was conducted using fermentation broths of Aspergillus niger with different pellet morphology. Copper extraction gradually increased with reduction in pellet diameter of A. niger. A large amount of compact smooth pellets with a diameter of 0.96 mm were obtained under the optimum fermentation conditions of 30℃, 0.8% inoculum size(OD600=0.1), 30% potato-sucrose contents, 65 h fermentation time. After 3 days of leaching, copper extraction reached 83.25%with the fermentation broths aforementioned at 30℃, 180 r·min?1.