CAJ | 학술논문

目的：探讨FTY720对卵巢癌细胞系OVCAR-3增殖的影响及其诱导凋亡的作用机制。方法应用不同浓度的FTY720作用于卵巢癌细胞系OVCAR-3，MTT法检测细胞生长抑制率，应用Annexin V-FITC／PI细胞凋亡双染试剂盒检测细胞凋亡率及分析细胞周期的变化，Western-blot法分析凋亡相关蛋白的变化。结果 FTY720对OVCAR-3卵巢癌细胞系增殖的抑制作用具有时间、剂量依赖性。随着FTY720作用时间的延长，OVCAR-3细胞凋亡数量增多，且G1期细胞比例逐渐上升。 Western-blot结果显示Caspase3和Caspase9都受到了激活。结论 FTY720可以诱导人卵巢癌细胞系OVCAR-3发生凋亡，并且激活内源性Caspase途径和诱导细胞发生G1期阻滞，这可能是FTY720抑制卵巢癌细胞生长的作用机制之一。
목적：탐토FTY720대란소암세포계OVCAR-3증식적영향급기유도조망적작용궤제。방법응용불동농도적FTY720작용우란소암세포계OVCAR-3，MTT법검측세포생장억제솔，응용Annexin V-FITC／PI세포조망쌍염시제합검측세포조망솔급분석세포주기적변화，Western-blot법분석조망상관단백적변화。결과 FTY720대OVCAR-3란소암세포계증식적억제작용구유시간、제량의뢰성。수착FTY720작용시간적연장，OVCAR-3세포조망수량증다，차G1기세포비례축점상승。 Western-blot결과현시Caspase3화Caspase9도수도료격활。결론 FTY720가이유도인란소암세포계OVCAR-3발생조망，병차격활내원성Caspase도경화유도세포발생G1기조체，저가능시FTY720억제란소암세포생장적작용궤제지일。
Objective To investigate the effect of FTY720 on the proliferation of OVCAR-3 ovarian cancer cells and its mechanism of apoptosis-inducing in vitro.Methods OVCAR-3 cells under different concentration of FTY720 were collected.The inhibition rates of cells were determined by MTT assay to evaluate the effect of FTY720.The percentage of apoptosis and cell cycle progression were detected by Annexin V-FITC/PI.Apoptosis related proteins were determined by western-blot.Results Growth inhibition and apoptosis induction were evoked by different concentrations of FTY720 in OVCAR-3 cells in time and concentration dependent manner.FTY720 greatly inhibited human ovarian cancer cell OVCAR-3 proliferation.This effect was associated with G1 phase cell cycle arrest and apoptosis.Wester-blot showed that caspase3 and caspase9 were activated.Conclusion FTY720 can inhibit the proliferation and induce OVCAR-3 cells apoptosis.Apoptosis induced by FTY720 may be regulated through G1 phase cell cycle arrest and the activation of endogenous caspase pathway.