畜牧与饲料科学
畜牧與飼料科學
축목여사료과학
ANIMAL HUSBANDRY AND FEED SCIENCE
2015年
1期
7-9
,共3页
猪圆环病毒2型%PCR%诊断
豬圓環病毒2型%PCR%診斷
저원배병독2형%PCR%진단
PCV-2%PCR%diagnosis
参照GenBank中已发表的猪圆环病毒2型（PCV-2）ORF2基因序列，设计合成1对引物，建立了检测猪场疑似病例临床样本中PCV-2的PCR方法。结果显示，PCV-2的ORF2扩增产物经琼脂糖凝胶电泳，呈现1条630 bp的特异性条带，与预期目的片段大小一致。应用该方法对安徽省3个猪场的30份病料进行了PCV-2检测，结果有9份检测出PCV-2，阳性率为30%。结果表明，该试验建立的PCR方法，是一种快速、灵敏、高效的PCV-2检测技术。
參照GenBank中已髮錶的豬圓環病毒2型（PCV-2）ORF2基因序列，設計閤成1對引物，建立瞭檢測豬場疑似病例臨床樣本中PCV-2的PCR方法。結果顯示，PCV-2的ORF2擴增產物經瓊脂糖凝膠電泳，呈現1條630 bp的特異性條帶，與預期目的片段大小一緻。應用該方法對安徽省3箇豬場的30份病料進行瞭PCV-2檢測，結果有9份檢測齣PCV-2，暘性率為30%。結果錶明，該試驗建立的PCR方法，是一種快速、靈敏、高效的PCV-2檢測技術。
삼조GenBank중이발표적저원배병독2형（PCV-2）ORF2기인서렬，설계합성1대인물，건립료검측저장의사병례림상양본중PCV-2적PCR방법。결과현시，PCV-2적ORF2확증산물경경지당응효전영，정현1조630 bp적특이성조대，여예기목적편단대소일치。응용해방법대안휘성3개저장적30빈병료진행료PCV-2검측，결과유9빈검측출PCV-2，양성솔위30%。결과표명，해시험건립적PCR방법，시일충쾌속、령민、고효적PCV-2검측기술。
In present study, a PCR method was established based on the ORF2 gene of PCV-2 published in GenBank to detect the PCV-2 in clinical samples from three pig farms. The results showed that a specific band with the anticipated size of 630 bp was obtained. A total of 30 clinical samples from three pig farms were detected for the presence of PCV-2 by the established PCR method and 9 of them were PCV-2 positive, the positive rate was 30%. It was indicated that the PCR method established in this study was a fast, sentitive and efficient strategy for the molecular diagnosis of PCV-2 infection in porcine farms.
