CAJ | 학술논문

为了解猪圆环病毒2型（PCV-2）在河南地区猪场的流行情况，采用常规方法对采集自河南省洛阳市某猪场病料中的PCV-2进行了分离，并对其全基因组进行了扩增、克隆和测序。结果表明，从患病仔猪的病料中分离到了1株PCV-2，命名为LN株，该毒株的全基因组序列大小为1767 bp。该试验结果为从分子水平上揭示PCV-2的致病特性，开发PCV-2的相关疫苗和诊断产品奠定了基础。
위료해저원배병독2형（PCV-2）재하남지구저장적류행정황，채용상규방법대채집자하남성락양시모저장병료중적PCV-2진행료분리，병대기전기인조진행료확증、극륭화측서。결과표명，종환병자저적병료중분리도료1주PCV-2，명명위LN주，해독주적전기인조서렬대소위1767 bp。해시험결과위종분자수평상게시PCV-2적치병특성，개발PCV-2적상관역묘화진단산품전정료기출。
In order to understand the prevalence of porcine circovirus type 2 (PCV-2) in pig farms in Henan Province,the clinical samples of a pig farm in Luoyang City of Henan Province were collected and were used for the isolation of PCV-2. Furthermore, the genome of the isolate was amplified, cloned and sequenced. The results showed that a PCV-2 strain were isolated from the samples and was named as LN. The size of the genome of LN strain was 1 767 bp. The results of the study will provide basis for revealing the pathogenic character of PCV-2 at molecular level and developing the vaccine and diagnostic products for PCV-2.