CAJ | 학술논문

目的：建立帕金森病抑郁（PDD）大鼠模型，观察中药柴甘解忧汤对其行为学及脑组织单胺递质的影响。方法：6－羟基多巴（6-OHDA）及孤养结合慢性持续不可预见温和应激法（CUMS）制备PDD模型。通过阿扑吗啡（APO）诱导旋转行为、体重测量、旷场试验及蔗糖偏嗜实验评定其行为学变化，并用酶联免疫法检测大鼠前额叶皮质、海马单胺神经递质含量。结果：模型组大鼠出现明显行为学异常，脑组织单胺递质含量明显降低，柴甘解忧汤组可显著改善其行为学改变并增加脑组织单胺递质含量。结论：6-OHDA及CUMS的造模方法可制备较理想的PDD模型；柴甘解忧汤可明显改善PDD大鼠的行为学异常，其抗 PDD 作用与增加脑组织单胺递质含量有关。
목적：건립파금삼병억욱（PDD）대서모형，관찰중약시감해우탕대기행위학급뇌조직단알체질적영향。방법：6－간기다파（6-OHDA）급고양결합만성지속불가예견온화응격법（CUMS）제비PDD모형。통과아복마배（APO）유도선전행위、체중측량、광장시험급자당편기실험평정기행위학변화，병용매련면역법검측대서전액협피질、해마단알신경체질함량。결과：모형조대서출현명현행위학이상，뇌조직단알체질함량명현강저，시감해우탕조가현저개선기행위학개변병증가뇌조직단알체질함량。결론：6-OHDA급CUMS적조모방법가제비교이상적PDD모형；시감해우탕가명현개선PDD대서적행위학이상，기항 PDD 작용여증가뇌조직단알체질함량유관。
Objective To build rat models and observe changes of behavior and monoamine of PDD. Methods The rats were injected with 6-OHDA and accepted CUMS for the establishment of PDD model , so as to assess its behavior changes and detect brain tissue monoamine contents by ELISA. Results Significant behavioral and monoamine abnormalities can be observed in model rats. Behavioral and monoamine improvement can be observed after treated with Chaigan Jieyou. Conclusion The PDD model can be established by 6-OHDA and CUMS. Chaigan Jieyou decoction may reach therapeutic purposes by increasing monoamine contents.