CAJ | 학술논문

目的：探讨乙型肝炎病毒（HBV）基本核心启动子（BCP）区突变与广西肝癌家族聚集的相关性。方法：从广西肝癌高发及无癌家族中配对选取 HBsAg 阳性成员各103例。采用聚合酶链反应（PCR）扩增BCP 区并测序分析。结果：BCP 区突变发生在前5位的热点位点为 T1762、A1764、G1775、V1753、G1803。单因素分析：HBV DNA≥105 copies／mL、T1762、A1764和V1753突变均与肝癌高发有关（P＜0．05）。多因素Logistic分析显示：HBV DNA≥105 copies／mL和 A1764突变是肝癌高发的独立危险因素。结论：HBV DNA水平、BCP区突变与广西肝癌家族聚集存在相关性。
목적：탐토을형간염병독（HBV）기본핵심계동자（BCP）구돌변여엄서간암가족취집적상관성。방법：종엄서간암고발급무암가족중배대선취 HBsAg 양성성원각103례。채용취합매련반응（PCR）확증BCP 구병측서분석。결과：BCP 구돌변발생재전5위적열점위점위 T1762、A1764、G1775、V1753、G1803。단인소분석：HBV DNA≥105 copies／mL、T1762、A1764화V1753돌변균여간암고발유관（P＜0．05）。다인소Logistic분석현시：HBV DNA≥105 copies／mL화 A1764돌변시간암고발적독립위험인소。결론：HBV DNA수평、BCP구돌변여엄서간암가족취집존재상관성。
Objective To explore the relationship between mutations in basic core promoter (BCP) of hepatitis B virus (HBV) and familial clustering of hepatocellular carcinoma (HCC) in Guangxi. Methods 153 pairs of members with HBsAg-positive were selected and matched from HCC high-incidence families and carcinoma-free families in Guangxi. The BCP genes were amplified and sequenced. Results The hotspot sites of the previous five mutations in BCP were T1762, A1764, G1775, V1753, G1803. In univariant analysis, HBV DNA≥105 copies/mL, T1762, A1764 and V1753 mutations were associated with the HCC high-incidence (P <0.05). The multivariate logistic analysis showed that HBV DNA≥105 copies/mL and A1764 were independent risk factors for it. Conclusion HBV DNA level, the mutations in BCP showed correlations with familial clustering of HCC in Guangxi.