CAJ | 학술논문

目的：探讨靛玉红减轻自发性蛛网膜下腔出血（SAH）小鼠早期脑损害的作用。方法：90只雄性C57BL／6J小鼠随机分为假手术组、SAH＋生理盐水组及SAH＋靛玉红组，用血管内刺破法建立SAH模型。干湿重法检测脑含水量，神经病学评分评价小鼠的神经功能损伤，TUNEL法检测出血侧脑皮层及海马凋亡神经元的变化。结果：SAH总病死率为23．3％，其中SAH＋生理盐水组病死率为24．1％，SAH＋靛玉红组病死率为21．8％，差异无统计学意义（P＞0．05）。神经病学评分，SAH＋生理盐水组24、72 h分别为11．13±2．45、13．81±2．46，SAH＋靛玉红组分别为12．55±2．11、16．32±1．09，各组间各时间点比较差异有统计学意义（P＜0．05）。在出血侧大脑半球，SAH＋靛玉红组脑含水量较SAH＋生理盐水组脑含水量减少明显[（77．57±0．35）％vs （78．12±0．47）％，P＜0．05]；SAH＋靛玉红组出血侧脑皮层及海马区TUNEL法检测阳性细胞与SAH＋生理盐水组比较明显减少（P＜0．05）。结论：靛玉红可减轻SAH后的脑水肿、抑制脑皮层和海马神经元的变性凋亡，明显改善SAH小鼠的神经功能损伤。
목적：탐토전옥홍감경자발성주망막하강출혈（SAH）소서조기뇌손해적작용。방법：90지웅성C57BL／6J소서수궤분위가수술조、SAH＋생리염수조급SAH＋전옥홍조，용혈관내자파법건립SAH모형。간습중법검측뇌함수량，신경병학평분평개소서적신경공능손상，TUNEL법검측출혈측뇌피층급해마조망신경원적변화。결과：SAH총병사솔위23．3％，기중SAH＋생리염수조병사솔위24．1％，SAH＋전옥홍조병사솔위21．8％，차이무통계학의의（P＞0．05）。신경병학평분，SAH＋생리염수조24、72 h분별위11．13±2．45、13．81±2．46，SAH＋전옥홍조분별위12．55±2．11、16．32±1．09，각조간각시간점비교차이유통계학의의（P＜0．05）。재출혈측대뇌반구，SAH＋전옥홍조뇌함수량교SAH＋생리염수조뇌함수량감소명현[（77．57±0．35）％vs （78．12±0．47）％，P＜0．05]；SAH＋전옥홍조출혈측뇌피층급해마구TUNEL법검측양성세포여SAH＋생리염수조비교명현감소（P＜0．05）。결론：전옥홍가감경SAH후적뇌수종、억제뇌피층화해마신경원적변성조망，명현개선SAH소서적신경공능손상。
Objective To investigate the inhibition effect of indirubin on early brain injury after spontaneous subarachnoid hemorrhage. Methods Ninety male adult C57BL/6J mice were randomly divided into 3 groups:sham group, SAH+vehicle group and SAH+Indirubin group. Subarachnoid hemorrhage model was established by endovascular puncture method, and the blood flow of bleeding cerebral hemisphere was measured by laser Doppler flowmeter. The wet-dry weighing method was performed to measure the brain water content.The neurological appraisal was evaluated by neurological score. The apoptosis of neuron in the cortex and hippocampus was examined by TUNEL staining. Results SAH total mortality was 23.3%. Mortality showed statistical difference between SAH+vehicle group (24.1%) and SAH+Indirubingroups (21.8%).The neurological scores of the SAH+vehicle group were 11.13 ± 2.45、13.81 ± 2.46 at 24, 72 h, which was higher than SAH+Indirubin group(12.55 ± 2.11、16.32 ± 1.09). There were significant difference between groups at the same time point(P<0.05). At the bleeding cerebral hemisphere, brain water content of SAH+ Indirubin group was (77.57 ± 0.35)%, which was better than SAH+vehicle group (78.12 ± 0.47)%(P<0.05). The TUNEL positive cells rate of Cortex and hippocampus in SAH+Indirubin group are lower than SAH+vehicle group (P<0.05). Conclusion Indirubin can relieve cerebral edema,reduce apoptosis of the cerebral cortex and hippocampal neurons, and improve early neurological function of SAH mice.