CAJ | 학술논문

目的：建立一种同时测定蜘蛛香药材中缬草三酯、乙酰缬草三酯及其降解产物baldrinal、11-ethoxyviburtinal含量的方法。方法：色谱柱：Agilent ZORBAX SB-C18柱（4.6 mm×250 mm，5μm）；流动相：水（A）-乙腈（B），梯度洗脱；流速：1 mL·min-1；柱温：25℃；检测波长：241 nm；进样量：10μL。结果：14个不同产地蜘蛛香药材中4种成分的含量差异较大。其中缬草三酯、乙酰缬草三酯、baldrinal的含量均以云南宝山蜘蛛香为最高，11-ethoxyviburtinal的含量以云南大理蜘蛛香为最高。结论：本研究所建立的方法精密度、重复性、稳定性良好，适用于蜘蛛香药材中4种成分的含量测定，为蜘蛛香的质量控制和开发利用提供依据。
목적：건립일충동시측정지주향약재중힐초삼지、을선힐초삼지급기강해산물baldrinal、11-ethoxyviburtinal함량적방법。방법：색보주：Agilent ZORBAX SB-C18주（4.6 mm×250 mm，5μm）；류동상：수（A）-을정（B），제도세탈；류속：1 mL·min-1；주온：25℃；검측파장：241 nm；진양량：10μL。결과：14개불동산지지주향약재중4충성분적함량차이교대。기중힐초삼지、을선힐초삼지、baldrinal적함량균이운남보산지주향위최고，11-ethoxyviburtinal적함량이운남대리지주향위최고。결론：본연구소건립적방법정밀도、중복성、은정성량호，괄용우지주향약재중4충성분적함량측정，위지주향적질량공제화개발이용제공의거。
This study was aimed to establish an HPLC method for simultaneous content determination of valtrate, acevaltrate, and their degradation products, which were baldrinal and 11-ethoxyviburtinal, in Valeriana jatamansi Jones. The separation and quantification of 4 constituents mentioned above were performed on an Agilent ZORBAX SB-C18 (4.6 mm×250 mm, 5 μm). The mobile phase consisted of water (A) - acetonitrile (B) with an optimized gradient program. The flow rate was 1 mL·min-1. The column temperature was set at 25℃. The wavelength was set at 241 nm. And the injection volume was 10μL. The results showed that among 14 different places of V. jatamansi, the 4 contents determined were different. The contents of valtrate, acevaltrate, and baldrinal in the Yunnan Baoshan Mount were the highest. And the content of 11-ethoxyviburtinal was the highest in Yunnan Dali. It was concluded that the method was with good precision, reproducibility and stability. And it was suitable for the determination of 4 valepotriates ingredients in V. jatamansi. It also provided references for the quality control and exploitation of V. jatamansi.