CAJ | 학술논문

目的：探讨老龄小鼠脑缺血后脑内血管上纤维连接蛋白（fibronectin, Fn）及其受体整合素α5β1和αVβ3的表达方式及其与血管新生间的关系。方法选取2月龄（青年）、18月龄（老龄）雄性C57BL/6小鼠为观察对象。以线栓法制备大脑中动脉栓塞模型，各年龄段分为假手术组（对照组）、脑缺血组，于模型成功后7 d，14 d行免疫荧光染色（各组n=5）。以CD31和Ki67的双重免疫荧光染色检测内皮细胞增殖，以每个视野中CD31阳性血管数代表血管密度。用CD31/Fn,CD31/ɑ5和CD31/β3双重免疫荧光染色确定Fn，α5β1和αVβ3在血管上的表达。结果缺血第7 d时老龄鼠半暗带区CD31/Ki67双阳性细胞数显著低于青年鼠（4.7±0.8/field, P＜0.05）；缺血7 d和14 d时老龄鼠半暗带区血管密度均比青年鼠显著降低（38.3±3.9/field,45±4.4/field,均P＜0.01）。同时，青年和老龄鼠脑缺血7 d后半暗带区血管上Fn，α5和β3整合素的表达较之于对照组均呈不同程度平行上调，而14 d时则趋于下降。而较之于青年鼠，老龄鼠缺血7 d后半暗带区Fn/α5/β3阳性血管数均显著降低（P＜0.05）。结论老龄小鼠脑缺血后血管新生缺陷可能与血管上Fn及其受体α5β1、αVβ3表达上调不足有关。
목적：탐토노령소서뇌결혈후뇌내혈관상섬유련접단백（fibronectin, Fn）급기수체정합소α5β1화αVβ3적표체방식급기여혈관신생간적관계。방법선취2월령（청년）、18월령（노령）웅성C57BL/6소서위관찰대상。이선전법제비대뇌중동맥전새모형，각년령단분위가수술조（대조조）、뇌결혈조，우모형성공후7 d，14 d행면역형광염색（각조n=5）。이CD31화Ki67적쌍중면역형광염색검측내피세포증식，이매개시야중CD31양성혈관수대표혈관밀도。용CD31/Fn,CD31/ɑ5화CD31/β3쌍중면역형광염색학정Fn，α5β1화αVβ3재혈관상적표체。결과결혈제7 d시노령서반암대구CD31/Ki67쌍양성세포수현저저우청년서（4.7±0.8/field, P＜0.05）；결혈7 d화14 d시노령서반암대구혈관밀도균비청년서현저강저（38.3±3.9/field,45±4.4/field,균P＜0.01）。동시，청년화노령서뇌결혈7 d후반암대구혈관상Fn，α5화β3정합소적표체교지우대조조균정불동정도평행상조，이14 d시칙추우하강。이교지우청년서，노령서결혈7 d후반암대구Fn/α5/β3양성혈관수균현저강저（P＜0.05）。결론노령소서뇌결혈후혈관신생결함가능여혈관상Fn급기수체α5β1、αVβ3표체상조불족유관。
Objective To investigate the relationship between the expression patterns of fibronectin (Fn) and its receptors-α5β1 and αVβ3 integrins and angiogenesis after cerebral ischemia in aged mice. Methods Both young (2 months) and aged (18 months) C57BL6 male mice were subject to middle cerebral artery occlusion. The brain endothelial cells (BECs) proliferation was assessed by using dual-immunofluorescent staning (IF) for Ki67 and CD31 and the vessel density was quantified by counting the number of CD31-positive vessels per field of view. The expression patterns of Fn,α5β1 and αVβ3 on blood vessels was assessed by using dual-IF for CD31/ɑ5,CD31/β3 and CD31/Fn. Results The number of CD31/Ki67 dual-positive cells in the penumbra at day 7 after cerebral ischemia of was significantly lower in the aged mice than in the young mice (4.7±0.8/field, P<0.05). The number of CD31-positive vessels in the penumbra at day 7 and 14 of was both significantly lower in aged mice than in the young mice (38.3±3.9/field, 45±4.4/field, both P<0.01). Furthermore, the expression of Fn, α5 and β3 on blood vessels in the penumbra at day 7 after cerebral ischemia was significantly lower in age mice than in the young mice (P<0.05). Conclusions The cerebral angiogenesis after ce?rebral ischemia is impaired in the aged stroked mice, which is associated with the low expression of fibronectin and its receptors-α5β1 andαVβ3 integrins on blood vessels.