CAJ | 학술논문

目的：建立藏药十味消食散的质量控制方法。方法：采用薄层色谱法进行定性鉴别，采用HPLC方法测定桂皮醛和胡椒碱的含量，色谱柱为Phenomenex C18柱（250 mm×4.6 mm，5μm）。结果：TLC法可鉴别石榴子、诃子的特征斑点；桂皮醛、胡椒碱的进样量分别在0.06-0.37μg、0.05-0.33μg范围内呈良好的线性关系，回归方程分别为Y=9.2732×106X-2.3482×105，r=0.9998；Y=7.315×103X-3.857，r=1.0000。结论：鉴别方法专属性强，定量方法准确，可操作性强，可对十味消食散的质量控制提供参考。
목적：건립장약십미소식산적질량공제방법。방법：채용박층색보법진행정성감별，채용HPLC방법측정계피철화호초감적함량，색보주위Phenomenex C18주（250 mm×4.6 mm，5μm）。결과：TLC법가감별석류자、가자적특정반점；계피철、호초감적진양량분별재0.06-0.37μg、0.05-0.33μg범위내정량호적선성관계，회귀방정분별위Y=9.2732×106X-2.3482×105，r=0.9998；Y=7.315×103X-3.857，r=1.0000。결론：감별방법전속성강，정량방법준학，가조작성강，가대십미소식산적질량공제제공삼고。
This study was aimed to establish a method for quality control of Tibetan medicine Shi-Wei Xiao-Shi-San (SWXSS). Thin layer chromatography (TLC) was established for the qualitative determination. Cinnamal and piperine were determined by HPLC. The separation was performed on Phenomenex C18 column (4.6 mm × 250 mm, 5μm). The results showed that TLC can be used in the identification of pomegranate seed and Terminalia chebula. The linear ranges of cinnamal and piperine were within the range of 0.06-0.37μg and 0.05-0.33μg, respectively. And the standard line was Y=9.273 2×106X-2.348 2×105, r=0.999 8;Y=7.315×103X-3.857, r=1.000 0. It was concluded that the identification method was specific, accurate and practical, which can be applied in the quality control of SWXSS.