CAJ | 학술논문

目的：测定葛根芩连片中主要活性成分（葛根素、黄芩苷和盐酸小檗碱）及其在复方多成分环境下，于不同pH磷酸缓冲液中的溶解度及油水分配系数。方法：采用HPLC法测定葛根素、黄芩苷和盐酸小檗碱在不同pH介质中的溶解度，以及在正辛醇-水和正辛醇-缓冲液体系中的油水分配系数。结果：葛根素、黄芩苷和盐酸小檗碱的溶解度与油水分配系数随pH的变化而变化，在复方多成分背景下也会发生改变。在pH为7.4时溶解度最大，葛根素为7.56 mg·mL-1，黄芩苷为17.07 mg·mL-1，盐酸小檗碱为3.57 mg·mL-1；3者油水分配系数P在pH为1.0时较大，葛根素为0.420（lgP=-0.38），黄芩苷为10.783（lgP=1.03），盐酸小檗碱为0.267（lgP=-0.57）。结论：葛根素、黄芩苷和盐酸小檗碱在pH为1.0时脂溶性均较好，推测在胃里吸收较好，在其它pH介质下脂溶性较低，推测脂溶性可能是影响其肠吸收的原因之一。
목적：측정갈근금련편중주요활성성분（갈근소、황금감화염산소벽감）급기재복방다성분배경하，우불동pH린산완충액중적용해도급유수분배계수。방법：채용HPLC법측정갈근소、황금감화염산소벽감재불동pH개질중적용해도，이급재정신순-수화정신순-완충액체계중적유수분배계수。결과：갈근소、황금감화염산소벽감적용해도여유수분배계수수pH적변화이변화，재복방다성분배경하야회발생개변。재pH위7.4시용해도최대，갈근소위7.56 mg·mL-1，황금감위17.07 mg·mL-1，염산소벽감위3.57 mg·mL-1；3자유수분배계수P재pH위1.0시교대，갈근소위0.420（lgP=-0.38），황금감위10.783（lgP=1.03），염산소벽감위0.267（lgP=-0.57）。결론：갈근소、황금감화염산소벽감재pH위1.0시지용성균교호，추측재위리흡수교호，재기타pH개질하지용성교저，추측지용성가능시영향기장흡수적원인지일。
This study was aimed to determine the solubility an d oil-water partition coefficient of main active com-ponents in Ge-Gen Qin-Lian (GGQL) Tablets (puerarin, baicalin and berberine hydrochloride) in phosphate buffer solution of different pH values and under the background of many components. Solubility of puerarin, baicalin and berberine hydrochloride in different medium pH, and oil-water partition coefficient of the octanol-water and oc-tanol-buffer system were determined by HPLC method. The results showed that the solubility and oil-water partition coefficient of puerarin, baicalin and berberine hydrochloride were varied with the change of pH, and varied under the background of components. At pH 7.4, the solubility was the biggest;puerarin was 7.56 mg·mL-1;baicalin was 17.07 mg·mL-1; berberine hydrochloride was 3.57 mg·mL-1. Oil-water partition coefficient P of these components at pH 1.0 was bigger;puerarin was 0.420 (lgP=-0.38);baicalin was 10.783 (lgP=1.03);berberine hydrochloride was 0.267 (lgP=-0.57). It was concluded that lipid solubility of puerarin, baicalin and berberine hydrochloride at pH 1.0 was better. It was speculated that better absorption in the stomach, and low lipid solubility under other pH. It was speculated that lipid solubility may be one of the reasons affecting the intestinal absorption.