世界科学技术-中医药现代化
世界科學技術-中醫藥現代化
세계과학기술-중의약현대화
WORLD SCIENCE AND TECHNOLOGY-MODERNIZATION OF TRADITIONAL CHINESE MEDICINE
2014年
12期
2740-2745
,共6页
丹参胶囊%脂溶性成分%水溶性成分%高效液相%含量测定
丹參膠囊%脂溶性成分%水溶性成分%高效液相%含量測定
단삼효낭%지용성성분%수용성성분%고효액상%함량측정
Danshen capsule%liposoluble component%water-soluble component%HPLC%content determination
目的：采用高效液相色谱法分别建立同时测定丹参胶囊中脂溶性成分二氢丹参酮I、隐丹参酮、丹参酮I及丹参酮IIA和水溶性成分丹参素、原儿茶醛、迷迭香酸、丹酚酸B及丹酚酸A的含量的方法。方法：脂溶性成分：采用Welch ultimate XB-C18（250 mm×4.6 mm，5μm）色谱柱，以乙腈-水为流动相进行梯度洗脱，流速1 mL·min-1，检测波长为270 nm。水溶性成分：采用Thermo Syncronis C18（250 mm×4.6 mm,5μm）色谱柱，以甲醇-乙腈-0.02%磷酸溶液为流动相进行梯度洗脱，流速1 mL·min-1，检测波长为286 nm。结果：丹参素、原儿茶醛、迷迭香酸、丹酚酸B、丹酚酸A、二氢丹参酮I、隐丹参酮、丹参酮I和丹参酮IIA分别在0.472-9.44μg（r=0.9998）、0.352-7.04μg（r=0.9999）、0.244-4.88μg（r=1.0000）、2.268-45.36μg（r=0.9999）、0.168-3.36μg（r=0.9999）、0.088-1.76μg（r=0.9999）、0.18-3.6μg（r=0.9999）、0.208-4.16μg（r=0.9999）和0.17-3.4μg（r=0.9999）呈良好的线性关系；加样回收率在97.48%-98.59%之间。结论：该方法稳定，重复性好，可用于丹参胶囊含量的测定。
目的：採用高效液相色譜法分彆建立同時測定丹參膠囊中脂溶性成分二氫丹參酮I、隱丹參酮、丹參酮I及丹參酮IIA和水溶性成分丹參素、原兒茶醛、迷迭香痠、丹酚痠B及丹酚痠A的含量的方法。方法：脂溶性成分：採用Welch ultimate XB-C18（250 mm×4.6 mm，5μm）色譜柱，以乙腈-水為流動相進行梯度洗脫，流速1 mL·min-1，檢測波長為270 nm。水溶性成分：採用Thermo Syncronis C18（250 mm×4.6 mm,5μm）色譜柱，以甲醇-乙腈-0.02%燐痠溶液為流動相進行梯度洗脫，流速1 mL·min-1，檢測波長為286 nm。結果：丹參素、原兒茶醛、迷迭香痠、丹酚痠B、丹酚痠A、二氫丹參酮I、隱丹參酮、丹參酮I和丹參酮IIA分彆在0.472-9.44μg（r=0.9998）、0.352-7.04μg（r=0.9999）、0.244-4.88μg（r=1.0000）、2.268-45.36μg（r=0.9999）、0.168-3.36μg（r=0.9999）、0.088-1.76μg（r=0.9999）、0.18-3.6μg（r=0.9999）、0.208-4.16μg（r=0.9999）和0.17-3.4μg（r=0.9999）呈良好的線性關繫；加樣迴收率在97.48%-98.59%之間。結論：該方法穩定，重複性好，可用于丹參膠囊含量的測定。
목적：채용고효액상색보법분별건립동시측정단삼효낭중지용성성분이경단삼동I、은단삼동、단삼동I급단삼동IIA화수용성성분단삼소、원인다철、미질향산、단분산B급단분산A적함량적방법。방법：지용성성분：채용Welch ultimate XB-C18（250 mm×4.6 mm，5μm）색보주，이을정-수위류동상진행제도세탈，류속1 mL·min-1，검측파장위270 nm。수용성성분：채용Thermo Syncronis C18（250 mm×4.6 mm,5μm）색보주，이갑순-을정-0.02%린산용액위류동상진행제도세탈，류속1 mL·min-1，검측파장위286 nm。결과：단삼소、원인다철、미질향산、단분산B、단분산A、이경단삼동I、은단삼동、단삼동I화단삼동IIA분별재0.472-9.44μg（r=0.9998）、0.352-7.04μg（r=0.9999）、0.244-4.88μg（r=1.0000）、2.268-45.36μg（r=0.9999）、0.168-3.36μg（r=0.9999）、0.088-1.76μg（r=0.9999）、0.18-3.6μg（r=0.9999）、0.208-4.16μg（r=0.9999）화0.17-3.4μg（r=0.9999）정량호적선성관계；가양회수솔재97.48%-98.59%지간。결론：해방법은정，중복성호，가용우단삼효낭함량적측정。
This study was aimed to establish a HPLC method for the content determination of liposoluble components, such as dihydrotanshione I, croptotanshinone, tanshinone I and tanshinone IIA, as well as the content determination of water-soluble components, such as danshensu, protocatechuic aldehyde, rosmarinc acid, salvianolic acid B, and salvianolic acid A in Danshen capsule simultaneously. For liposoluble components, the determination was performed on a Welch ultimate XB-C18 column (250 mm × 4.6 mm, 5 μm) by gradient elution using acetonitrile-water as the mobile phase. The flow rate was 1 mL·min-1. And the detection wavelength was set at 270 nm. For water-soluble components, the determination was performed on a Thermo Syncronis C18 column (250 mm × 4.6 mm, 5μm) by gradient elution using methanol-acetonitrile-0.02%phosphoric acid as the mobile phase. The flow rate was 1 mL·min-1. And the detection wavelength was set at 286 nm. The results showed that there were good linear relationships between components of peak areas and the ranges were 0.472-9.44 μg (r = 0.999 8) for danshensu, 0.352-7.04 μg (r = 0.999 9) for protocatechuic aldehyde, 0.244-4.88 μg (r = 1.000 0) for rosmarinc acid, 2.268-45.36 μg (r = 0.999 9) for salvianolic acid B, 0.168-3.36 μg (r = 0.999 9) for salvianolic acid A, 0.088-1.76 g(r=0.999 9) for dihydrotanshione I, 0.18-3.6μg (r=0.999 9) for croptotanshinone, 0.208-4.16μg (r=0.999 9) for tanshinone I, and 0.17-3.4μg (r=0.999 9) for tanshinone IIA. Average recoveries of the method were between 97.48%and 98.59%. It was concluded that the analysis was stable and reproducible, which can be used as a method for the analysis of Danshen capsule.
