CAJ | 학술논문

目的：初步探索不同给药时间对7种抗抑郁药在小鼠体内抗抑郁效果的影响。方法雄性小鼠随机分为8组，每组各36只：文拉法辛组（75 mg/kg）、舍曲林组（20 mg/kg）、氟西汀组（20 mg/kg）、多塞平组（15 mg/kg）、米氮平组（15 mg/kg）、西酞普兰（40 mg/kg）、曲唑酮组（50 mg/kg）和对照组（生理盐水）。分别在明相（9：00、13：00、17：00）和暗相（21：00、1：00、5：00）6个时间点前30 min采用相应药物灌胃给药，每个时间点灌胃6只小鼠，给药体积为0.1 mL/10 g，30min后采用强迫游泳实验测定这7种抗抑郁药抗抑郁作用效果。结果在各时间点上，与对照组相比，文拉法辛组和舍曲林组小鼠的不动时间均缩短（均P<0.05）。文拉法辛、氟西汀、米氮平、多塞平组在暗相的不动时间短于明相（均P<0.05）；而舍曲林组小鼠在暗相晚期（5：00）和明相早期（9：00）的不动时间较其他时间点缩短（P<0.05）。结论大多数抗抑郁药抗抑郁作用效果存在24 h节律，但不同抗抑郁药其节律模式并非完全一致，有待进一步开展这些抗抑郁药时辰药理学的作用机制及临床研究。
목적：초보탐색불동급약시간대7충항억욱약재소서체내항억욱효과적영향。방법웅성소서수궤분위8조，매조각36지：문랍법신조（75 mg/kg）、사곡림조（20 mg/kg）、불서정조（20 mg/kg）、다새평조（15 mg/kg）、미담평조（15 mg/kg）、서태보란（40 mg/kg）、곡서동조（50 mg/kg）화대조조（생리염수）。분별재명상（9：00、13：00、17：00）화암상（21：00、1：00、5：00）6개시간점전30 min채용상응약물관위급약，매개시간점관위6지소서，급약체적위0.1 mL/10 g，30min후채용강박유영실험측정저7충항억욱약항억욱작용효과。결과재각시간점상，여대조조상비，문랍법신조화사곡림조소서적불동시간균축단（균P<0.05）。문랍법신、불서정、미담평、다새평조재암상적불동시간단우명상（균P<0.05）；이사곡림조소서재암상만기（5：00）화명상조기（9：00）적불동시간교기타시간점축단（P<0.05）。결론대다수항억욱약항억욱작용효과존재24 h절률，단불동항억욱약기절률모식병비완전일치，유대진일보개전저사항억욱약시신약이학적작용궤제급림상연구。
Objective To explore the influence of different administration time on antidepressant effect of seven clinical common antidepressants. Methods Male mice were randomly divided into eight groups:venlafaxine (75 mg/kg), sertraline (20 mg/kg), fluoxetine (20 mg/kg), doxepin (15 mg/kg), mirtazapine (15 mg/kg), citalopram (40 mg/kg), trazodo?ne (50 mg/kg) and control (saline) groups. Each group contained 36 mice. Drugs were administered to 6 mice per group 30 min before forced swimming test at the 6 time points (9:00, 13:00 and 17:00 as light phase and 21:00, 1:00 and 5:00 as dark phase). Forced swimming test was applied to determine the influence of dosing time on anti-immobility effect of seven antidepressants at each time point. Results Immobility time in venlafaxine group and sertraline group significant?ly decreased compared with that of control group at all time points(all P<0.05). Moreover, anti-immobility effects of ven?lafaxine, fluoxetine, mirtazapine and doxepin were better during the dark phase than during the light phase (all P<0.05). In addition, immobility time in sertraline group decreased at the late part of dark phase (5:00) and the early part of light phase (9:00) compared with other phases (P<0.05). Conclusions Most antidepressants show 24-h rhythm dependent an?ti-immobility effects, but rhythmic patterns are not completely consistent among different antidepressants. Further study is needed to explore the chronopharmacological mechanism and clinical applications of these antidepressants.