CAJ | 학술논문

目的：探讨甲强龙（11β,17α,21-三羟基-6α-甲基孕甾-1,4-二烯-3,20二酮-21-琥珀酸钠）对大鼠脊髓损伤（spinal cord injury，SCI）后血-脊髓屏障（blood spinal cord barrier, BSCB）通透性及紧密连接蛋白claudin-5表达的影响。方法参照改良Allen’s法建立大鼠脊髓损伤模型。分为假手术组（n=50）、脊髓损伤组（n=50）、甲强龙预处理组（n=50）。建模后不同时间点（12h、1d、3d、5d、7d）各组随机取5只大鼠检测BSCB通透性，取胸段脊髓损伤区组织经RT-PCR、Western blot方法检测claudin-5表达。结果本实验制模成功率84.0%；脊髓损伤组各时间点EB含量较假手术组显著增高（F=27.732，P均＜0.05）。甲强龙预处理组各时间点EB含量较脊髓损伤组显著降低（F=48.149，P均＜0.05）；脊髓损伤组各时间点claudin-5 mRNA表达较假手术组显著降低（F=12.248，P均＜0.05）。甲强龙预处理组各时间点claudin-5 mRNA表达较脊髓损伤组显著升高（F=15.316，P均＜0.05）；脊髓损伤组各时间点claudin-5表达较假手术组显著降低（F=18.108，P均＜0.05）。甲强龙预处理组各时间点claudin-5表达较脊髓损伤组显著升高（F=20.247，P均＜0.05）。结论上调claudin-5表达可能是甲强龙改善大鼠脊髓损伤后血-脊髓屏障通透性的途径之一。
목적：탐토갑강룡（11β,17α,21-삼간기-6α-갑기잉치-1,4-이희-3,20이동-21-호박산납）대대서척수손상（spinal cord injury，SCI）후혈-척수병장（blood spinal cord barrier, BSCB）통투성급긴밀련접단백claudin-5표체적영향。방법삼조개량Allen’s법건립대서척수손상모형。분위가수술조（n=50）、척수손상조（n=50）、갑강룡예처리조（n=50）。건모후불동시간점（12h、1d、3d、5d、7d）각조수궤취5지대서검측BSCB통투성，취흉단척수손상구조직경RT-PCR、Western blot방법검측claudin-5표체。결과본실험제모성공솔84.0%；척수손상조각시간점EB함량교가수술조현저증고（F=27.732，P균＜0.05）。갑강룡예처리조각시간점EB함량교척수손상조현저강저（F=48.149，P균＜0.05）；척수손상조각시간점claudin-5 mRNA표체교가수술조현저강저（F=12.248，P균＜0.05）。갑강룡예처리조각시간점claudin-5 mRNA표체교척수손상조현저승고（F=15.316，P균＜0.05）；척수손상조각시간점claudin-5표체교가수술조현저강저（F=18.108，P균＜0.05）。갑강룡예처리조각시간점claudin-5표체교척수손상조현저승고（F=20.247，P균＜0.05）。결론상조claudin-5표체가능시갑강룡개선대서척수손상후혈-척수병장통투성적도경지일。
Objective To study the effects of methylprednisolone on the permeability of blood-spinal cord barrier (BSCB) and claudin-5 expression after spinal cord injury in rats. Methods The rat model of spinal cord injury was estab?lished using modified Allen method. SD rats were randomly divided into sham-operated group, spinal cord injury group and methylprednisolone pretreatment group. The permeability of BSCB and expression of claudin–5 were assessed at 12 h, 1, 3, 5, and 7 d after the onset of spinal cord injury (five animals per each time point). RT-PCR and Western blot were used to detect the expression of claudin-5. Results The success rate of the model was 84.0%. EB content was sig?nificantly higher in spinal cord injury group than in sham-operated group at each time point (F value 27.732,P<0.05). EB content was lower in methylprednisolone pretreatment group than in spinal cord injury group (F value 48.149,P<0.05). The mRNA expression of claudin-5 was lower in spinal cord injury group than sham-operated group at each time point (F value 12.248,P<0.05). The mRNA expression of claudin–5 was higher in methylprednisolone pretreatment group than in spinal cord injury group at each time point (Fvalue 15.316,P<0.05). The protein expression of claudin-5 was lower in spinal cord injury group than in sham operated group at each time point (Fvalue 18.108,P<0.05). The pro?tein expression of Claudin-5 was higher in methylprednisolone pretreatment group than spinal cord injury group at each time point (F value 20.247,P<0.05). Conclusions Methylprednisolone improves permeability of BSCB after spinal cord injury probably through enhancing claudin-5 expression in rats. <br> was lower in spinal cord injury group than in sham operated group at each time point (Fvalue 18.108,P<0.05). The pro?tein expression of Claudin-5 was higher in methylprednisolone pretreatment group than spinal cord injury group at each time point (F value 20.247,P<0.05). Conclusions Methylprednisolone improves permeability of BSCB after spinal cord injury probably through enhancing claudin-5 expression in rats.