感染、炎症、修复
感染、炎癥、脩複
감염、염증、수복
INFECTION， INFLAMMATION， REPAIR
2014年
4期
195-198
,共4页
郑金光%胡森%李芳菲%张聪%高明娟%王晓娜%姚龙强%白晓东
鄭金光%鬍森%李芳菲%張聰%高明娟%王曉娜%姚龍彊%白曉東
정금광%호삼%리방비%장총%고명연%왕효나%요룡강%백효동
伏立诺他%组蛋白去乙酰化酶抑制剂%烫伤%器官功能%血流量
伏立諾他%組蛋白去乙酰化酶抑製劑%燙傷%器官功能%血流量
복립낙타%조단백거을선화매억제제%탕상%기관공능%혈류량
Suberoylanilide hydroxamic acid%Histone deacetylase inhibitor%Burns%Organ function%Blood flow
目的：：探讨组蛋白去乙酰化酶抑制剂伏立诺他(SAHA)对50%TBSA Ⅲ度烫伤大鼠器官功能及脏器血流量的影响。方法：雄性 SD大鼠48只,体重240~260 g,随机分为3组：①单烫组,于100℃水浴中背部浸泡15 s、腹部浸泡8 s,烫伤后,立即腹腔内注射0.25 ml 生理盐水；②SAHA组,烫伤后立即腹腔内注射0.25 ml SAHA注射液(7.5 mg/kg,溶于4 ml 生理盐水中)；③假烫组,37℃水浴浸泡后腹腔内注射0.25 ml 生理盐水。于伤后3 h和6 h采用多普勒血流仪测定肝、肾及小肠黏膜血流量,取腹主动脉血检测血清丙氨酸转氨酶(ALT)含量、肌酐(Cr)含量、肌酸激酶同工酶(CK-MB)活性、神经特异性烯醇化酶(NSE)含量和二胺氧化酶(DAO)活性。结果：伤后3 h,SAHA组肝、肾和小肠黏膜血流量显著高于单烫组(P均<0.05)；SAHA组6 h小肠黏膜血流量也显著高于单烫组(P<0.05)。SAHA 组伤后3 h Cr、NSE 含量和 DAO 活性显著低于单烫组(P 均<0.05),伤后6 h CK-MB、NSE 含量和 DAO 活性均显著低于单烫组(P 均<0.05)。结论：SAHA 能改善50%TBSAⅢ度烫伤大鼠器官功能指标以及腹腔脏器血流量,保护重要脏器功能。
目的：：探討組蛋白去乙酰化酶抑製劑伏立諾他(SAHA)對50%TBSA Ⅲ度燙傷大鼠器官功能及髒器血流量的影響。方法：雄性 SD大鼠48隻,體重240~260 g,隨機分為3組：①單燙組,于100℃水浴中揹部浸泡15 s、腹部浸泡8 s,燙傷後,立即腹腔內註射0.25 ml 生理鹽水；②SAHA組,燙傷後立即腹腔內註射0.25 ml SAHA註射液(7.5 mg/kg,溶于4 ml 生理鹽水中)；③假燙組,37℃水浴浸泡後腹腔內註射0.25 ml 生理鹽水。于傷後3 h和6 h採用多普勒血流儀測定肝、腎及小腸黏膜血流量,取腹主動脈血檢測血清丙氨痠轉氨酶(ALT)含量、肌酐(Cr)含量、肌痠激酶同工酶(CK-MB)活性、神經特異性烯醇化酶(NSE)含量和二胺氧化酶(DAO)活性。結果：傷後3 h,SAHA組肝、腎和小腸黏膜血流量顯著高于單燙組(P均<0.05)；SAHA組6 h小腸黏膜血流量也顯著高于單燙組(P<0.05)。SAHA 組傷後3 h Cr、NSE 含量和 DAO 活性顯著低于單燙組(P 均<0.05),傷後6 h CK-MB、NSE 含量和 DAO 活性均顯著低于單燙組(P 均<0.05)。結論：SAHA 能改善50%TBSAⅢ度燙傷大鼠器官功能指標以及腹腔髒器血流量,保護重要髒器功能。
목적：：탐토조단백거을선화매억제제복립낙타(SAHA)대50%TBSA Ⅲ도탕상대서기관공능급장기혈류량적영향。방법：웅성 SD대서48지,체중240~260 g,수궤분위3조：①단탕조,우100℃수욕중배부침포15 s、복부침포8 s,탕상후,립즉복강내주사0.25 ml 생리염수；②SAHA조,탕상후립즉복강내주사0.25 ml SAHA주사액(7.5 mg/kg,용우4 ml 생리염수중)；③가탕조,37℃수욕침포후복강내주사0.25 ml 생리염수。우상후3 h화6 h채용다보륵혈류의측정간、신급소장점막혈류량,취복주동맥혈검측혈청병안산전안매(ALT)함량、기항(Cr)함량、기산격매동공매(CK-MB)활성、신경특이성희순화매(NSE)함량화이알양화매(DAO)활성。결과：상후3 h,SAHA조간、신화소장점막혈류량현저고우단탕조(P균<0.05)；SAHA조6 h소장점막혈류량야현저고우단탕조(P<0.05)。SAHA 조상후3 h Cr、NSE 함량화 DAO 활성현저저우단탕조(P 균<0.05),상후6 h CK-MB、NSE 함량화 DAO 활성균현저저우단탕조(P 균<0.05)。결론：SAHA 능개선50%TBSAⅢ도탕상대서기관공능지표이급복강장기혈류량,보호중요장기공능。
Objective:To investigate the protective effects of histone deacetylase inhibitor suberoylanilide hydroxamic acid (SAHA)against injury to organ function and visceral blood flow in rats with 50%total body surface area (TBSA)full-thick-ness scald injury.Methods:Forty-eight male SD rats,weighting 240-260 g,were randomly divided into three groups. In scald group,rats were subj ected to scald inj ury by immersing the back of the trunk for 1 5 seconds and the abdomen for 8 seconds in 100°C water,resulting in full-thickness injury involving 50% TBSA,then they received 0.25 ml of normal saline (NS) intraperitoneally. In SAHA group,rats were subj ected to same extent of inj ury,but they received an intraperitoneal inj ection of 0.25 ml of SAHA (7.5 mg/kg,dissolved in 4 ml of NS). In sham-scald group,rats were treated with 37 ℃ water,and then received 0.25ml of NS intraperitoneally. Blood flow of liver,kidney and small intestine was measured with Doppler blood flow meter. Abdominal aorta blood was drawn for measuring the levels of alanine aminotransferase (ALT),creatinine (Cr),creatine kinase MB (CK-MB),neuron specific enolase (NSE),and the activity of diamine oxidase (DAO)3 and 6 hours after inj ury. Results:The blood flow of liver,kidney,and intestine in SAHA group was significantly richer than that of the scald group 3 hours after burns(all P<0.05). The blood flow of the intestine in SAHA group was still higher signifi-cantly compared with that of scald group 6 hours after burn (all P<0.05).The contents of Cr,NSE and the activity of DAO in SAHA group were significantly lower than those of the sham-scald group 3 hours after burn (all P<0.05),and the con-tents of CK-MB,NSE and the activity of DAO in SAHA group were significantly lower than those of the scald group 6 hours after burns (all P<0.05).Conclusions:SAHA can improve the organ functions and intestinal blood flow after an exten-sive deep burn,and it protects the function of vital organs.
