CAJ | 학술논문

目的：寻找活体鸡胚操作时的最佳背景染料及其应用条件。方法比较不同染料对鸡胚发育的影响及背景色效果与持续时间，选择最优者，并摸索其最佳浓度与剂量。结果鸵鸟墨水注射后鸡胚存活率最高[76.9%（30/39）]，背景色好，持续时间长，并且采用0.2ml/4ml浓度、≤0.3ml剂量综合效果最优。结论鸵鸟墨水用于活体鸡胚操作时的背景染料效果佳。
목적：심조활체계배조작시적최가배경염료급기응용조건。방법비교불동염료대계배발육적영향급배경색효과여지속시간，선택최우자，병모색기최가농도여제량。결과타조묵수주사후계배존활솔최고[76.9%（30/39）]，배경색호，지속시간장，병차채용0.2ml/4ml농도、≤0.3ml제량종합효과최우。결론타조묵수용우활체계배조작시적배경염료효과가。
Objective To explore the best background dying and its application on chick embryos in vivo. Method se?lect the best one after comparising survival rate , background color and duration of different kinds of background dyings on chick embryo, and explore its optimal concentration and measurement. Results the survival rate of ostrich ink was the highest one[76.9%(30/39)], and its background color is clear and durative, the concentration of 0.2ml/4ml and less than 0.3ml dose make optimal effect. Conclusion the ostrich ink is the optimal selection for background dying on chick embryos in vivo .