CAJ | 학술논문

目的：比较腹腔注射维生素D3（ VD3）1、2、3周后实行球囊手术对动脉粥样硬化形成的影响，探寻大鼠动脉粥样硬化造模的优化方法。方法选取雄性SD大鼠36只，随机分为正常组、模型组1、模型组2、模型组3，正常组6只，其余每组10只。对照组饲喂普通饲料，模型组1、2、3在实验开始时饲喂高脂饲料并腹腔注射VD340万IU／kg，分别于1、2、3周后行左侧颈总动脉球囊损伤手术，术后第0、2周再注射VD310万IU／kg。手术4周后，处理动物，测定TG、TC、HDL－C、LDL－C水平，检测血清中炎症因子hsCRP、IL－6、TNFα含量，观察HE染色的胸主动脉病理变化，分析血管壁厚度、斑块面积（PA）、血管横截面积（CVA）及校正斑块面积（PA／CVA）。结果与正常对照组相比，模型组2、3中TC、LDL－C含量显著升高（P＜0．05）；模型组1、2、3的hsCRP、IL－6及TNFα水平较对照组明显升高（P＜0．05），模型组3的hsCRP、IL－6及TNFα水平明显高于模型组1（P＜0．05）；病理观察显示模型组1、2、3均出现不同程度的AS斑块，血管壁厚度及PA／CVA均显著大于对照组（ P＜0．05）；模型组3有大量脂质泡沫沉积，而且PA、CVA及PA／CVA相比于模型组1、2均明显增加（ P＜0．05）。结论大鼠在高脂饮食和VD3腹腔注射基础上于3周后行颈总动脉球囊损伤手术，是诱导动脉粥样硬化模型的理想优化方法。
목적：비교복강주사유생소D3（ VD3）1、2、3주후실행구낭수술대동맥죽양경화형성적영향，탐심대서동맥죽양경화조모적우화방법。방법선취웅성SD대서36지，수궤분위정상조、모형조1、모형조2、모형조3，정상조6지，기여매조10지。대조조사위보통사료，모형조1、2、3재실험개시시사위고지사료병복강주사VD340만IU／kg，분별우1、2、3주후행좌측경총동맥구낭손상수술，술후제0、2주재주사VD310만IU／kg。수술4주후，처리동물，측정TG、TC、HDL－C、LDL－C수평，검측혈청중염증인자hsCRP、IL－6、TNFα함량，관찰HE염색적흉주동맥병리변화，분석혈관벽후도、반괴면적（PA）、혈관횡절면적（CVA）급교정반괴면적（PA／CVA）。결과여정상대조조상비，모형조2、3중TC、LDL－C함량현저승고（P＜0．05）；모형조1、2、3적hsCRP、IL－6급TNFα수평교대조조명현승고（P＜0．05），모형조3적hsCRP、IL－6급TNFα수평명현고우모형조1（P＜0．05）；병리관찰현시모형조1、2、3균출현불동정도적AS반괴，혈관벽후도급PA／CVA균현저대우대조조（ P＜0．05）；모형조3유대량지질포말침적，이차PA、CVA급PA／CVA상비우모형조1、2균명현증가（ P＜0．05）。결론대서재고지음식화VD3복강주사기출상우3주후행경총동맥구낭손상수술，시유도동맥죽양경화모형적이상우화방법。
Objective To compare the effects of forming atherosclerosis by conducting ballon injury operation after 1th, 2th and 3th week of Vitamin D3(VD3) i.p., exploring the best method for atherosclerosis modeling .Methods 36 male rats were selected for balloon-injured carotid artery .SD rats were divided into 4 groups randomly:control group ( n=6), Model group1 (n=10), Model group2(n=10), Model group3 (n=10).Control group were fed up with common diet.Model groups were fed up with high-fat diet and injected 4.0 ×105 IU/kg VD3 through enterocoelia in the beginning , followed by the balloon-injured left carotid artery operation after 1th, 2th and 3th week respectively and 1.0 ×105 IU/kg VD3injection at 0th, 2th week after operation.The rats were killed at 4th week after operation.The serum levels of TG, TC, HDL-C and LDL-C were checked .ELISA was used to detect the content of hsCRP , IL-6 and TNFα.HE staining was used to observe the pathological changes in the thoracic aorta , and the thoracic aorta thickness , plaque area ( PA) , cross-sectional area of vessel ( CVA) and the ratio of PA to CVA ( PA/CVA) were analyzed .Results After 4 weeks of operation , levels of TC and LDL-C were significantly increased in Model group 2 and 3 compared with that of the control group ( P<0.05).Furthermore, contents of hsCRP, IL-6 and TNFαof model groups were also seriously higher than that of the control group (P<0.05), and that of Model group 3 were the highest.Typical AS plagues were observed in different degrees in model groups, and thoracic aorta thickness and PA/CVA were obviously increased than that of control group (P<0.05). Model group 3 turned out masses of lipid foam cells accumulated , and PA, CVA and PA/CVA were significantly increased than that of Model group2 or 3.Conclusion The AS model can be established successfully in rats with ballon injury after 3 weeks of high-fat diet plus VD3 i.p., which is the ideal method to induced atherosclerosis model .