CAJ | 학술논문

目的：观察大鼠慢性炎性痛时背根神经节（ dorsal root ganglion ， DRG ）μ型阿片受体（ mu opioid receptor，MOR）表达的变化及炎症局部注射MOR激动剂和拮抗剂对痛阈的干预作用，探讨MOR在慢性炎性疼痛中的作用。方法足底注射弗氏完全佐剂（complete freund’s adjuvant，CFA）制备大鼠慢性炎性痛模型，采用免疫组化法检测DRG MOR阳性细胞的表达；经大鼠足跖背部分别注射MOR激动剂、拮抗剂，采用辐射热法检测给药前后大鼠痛阈的变化。结果足底注射CFA诱导出大鼠慢性炎性痛，在造模后第18天，痛阈依然低于正常对照组；免疫组化结果表明，与正常对照组相比，CFA大鼠DRG MOR阳性细胞表达增多（ P ＜0．01）；足跖背部注射MOR激动剂减轻CFA大鼠的疼痛，对正常大鼠痛阈无影响；足跖背部注射MOR拮抗剂加重CFA大鼠的疼痛，对正常大鼠痛阈无影响。结论 DRG神经元MOR在慢性炎性痛时表达上调，参与慢性炎性痛的外周调控，可能有助于防止慢性痛的进一步加重。
목적：관찰대서만성염성통시배근신경절（ dorsal root ganglion ， DRG ）μ형아편수체（ mu opioid receptor，MOR）표체적변화급염증국부주사MOR격동제화길항제대통역적간예작용，탐토MOR재만성염성동통중적작용。방법족저주사불씨완전좌제（complete freund’s adjuvant，CFA）제비대서만성염성통모형，채용면역조화법검측DRG MOR양성세포적표체；경대서족척배부분별주사MOR격동제、길항제，채용복사열법검측급약전후대서통역적변화。결과족저주사CFA유도출대서만성염성통，재조모후제18천，통역의연저우정상대조조；면역조화결과표명，여정상대조조상비，CFA대서DRG MOR양성세포표체증다（ P ＜0．01）；족척배부주사MOR격동제감경CFA대서적동통，대정상대서통역무영향；족척배부주사MOR길항제가중CFA대서적동통，대정상대서통역무영향。결론 DRG신경원MOR재만성염성통시표체상조，삼여만성염성통적외주조공，가능유조우방지만성통적진일보가중。
Objective To investigate the change of mu opioid receptor ( MOR) in dorsal root ganglion ( DRG) in rat chronic inflammatory pain model and the effect of MOR agonist and antagonis tintraplantarly ( i.pl.) injected on pain threshold, so as to determine the role of peripheral MOR in chron in inflammatory pain .Methods Chronicin flammatory pain model was established by i .pl.injection of CFA in rats.The expression of MOR in DRG was detected by immunohistochemistry .Pain threshold before and after i .pl.injection of MOR agonist and antagonist was measured by radiant heat method .Results Rats suffered from an intraplantar injection of CFA developed chronic inflammatory pain , and the painthreshold still reduced on 18 day after CFA injection compared to that in the normal group . Immunohistochemistry staining revealed that compared with the normal group , the expression of MOR in DRG of CFA rats was increased ( P<0.01 ) .After the paw dorsal surface injection of MOR agonist , the pain threshold of CFA rats was increased, while that of normal rats exhibited no significant change .After the paw dorsal surface injection of MOR antagonist, the pain threshold of CFA rats was reduced , while that of normal rats had no significant change .Conclusion Under chronic inflammatory pain condition , DRG MOR expressionis enhanced , which participates in the regulation of chronic inflammatory pain , and may contribute to the prevention of further more serious pain .