CAJ | 학술논문

目的：观察敦煌平胃丸对胃癌前病变（PLGC）模型大鼠胃黏膜组织 Notch 信号通路中 Notch2和 Jagged1表达的影响。方法：采用复合法建立 PLGC 大鼠模型，将32只大鼠随机分为4组，每组8只。敦煌平胃丸组用敦煌平胃丸浸膏按每日生药96 g ／kg 掺入饲料中喂养给药，共计12周。观察大鼠胃黏膜病理组织学变化；采用 Western Blot 检测 Notch2和 Jagged1蛋白表达；采用 RT －PCR 检测Notch2和 Jagged1mRNA 的表达。结果：PLGC 大鼠胃黏膜组织 Notch2与 Jagged1mRNA 及蛋白表达较正常大鼠胃黏膜组织均明显上调（P ＜0.05）。敦煌平胃丸能明显改善 PLGC 大鼠胃黏膜腺体萎缩、肠化增生，下调 PLGC 大鼠胃黏膜组织中 Notch2与 Jagged1表达水平（P ＜0.05）。结论：Notch2、Jagged1激活了 Notch 信号通路，在 PLGC 发生发展中有重要意义。敦煌平胃丸治疗 PLGC 可能与下调 Notch 信号通路中 Notch2与 Jagged1表达有关。
목적：관찰돈황평위환대위암전병변（PLGC）모형대서위점막조직 Notch 신호통로중 Notch2화 Jagged1표체적영향。방법：채용복합법건립 PLGC 대서모형，장32지대서수궤분위4조，매조8지。돈황평위환조용돈황평위환침고안매일생약96 g ／kg 참입사료중위양급약，공계12주。관찰대서위점막병리조직학변화；채용 Western Blot 검측 Notch2화 Jagged1단백표체；채용 RT －PCR 검측Notch2화 Jagged1mRNA 적표체。결과：PLGC 대서위점막조직 Notch2여 Jagged1mRNA 급단백표체교정상대서위점막조직균명현상조（P ＜0.05）。돈황평위환능명현개선 PLGC 대서위점막선체위축、장화증생，하조 PLGC 대서위점막조직중 Notch2여 Jagged1표체수평（P ＜0.05）。결론：Notch2、Jagged1격활료 Notch 신호통로，재 PLGC 발생발전중유중요의의。돈황평위환치료 PLGC 가능여하조 Notch 신호통로중 Notch2여 Jagged1표체유관。
Objective To observe the Dunhuang pingwei pill on the expression of Notch2 and Jagged1 in the Notch signal pathway in rats with precancerous lesions of gastric cancer.Methods To establish the rat model of PLGC by using composite method,32 rats were randomly divided into 4 groups,8 rats in each group,Dunhuang pingwei pill group were fed with Dunhuang pingwei concrete ac-cording to daily crude drug 96 g/kg soak in feedstuff ,a total of 12 weeks.Observe changes in gastric mucosa pathological histology in rats.Using Western Blot to detect protein expression of Notch2 and Jagged1,the transcriptions of Notch2 and Jagged1mRNA were demonstrated by RT -PCR technique.Result Compared to the normal rats,Notch2 and Jagged1mRNA、protein expression were sig-nificantly up -regulated in gastric mucosa histology in rats with PLGC .(P <0.05).Dunhuang pingwei pill can obviously improve the gastric mucosa gland atrophy,intestinal metaphases and hyperplasia ,down -regulate the expression level of Jagged1andNotch2 in gastric mucosa tissue in rats with PLGC .(P <0.05).Conclusion Notch2,Jagged1 activates Notch signaling pathways in PLGC,it is important to the development of PLGC.Dunhuang pingwei pill in the treatment of PLGC maybe associated with down -regulating ex-pression of Notch2 and Jagged1 in Notch signal pathway.