CAJ | 학술논문

以粘红酵母( Rhodotorula glutinis)为出发菌株,采用常压室温等离子体对其进行诱变。得到最佳诱变条件为120 W功率下处理60 s,致死率达90%。诱变后,采用苏丹黑B染色法筛选出1株高产油脂突变株LA1,与出发菌株相比较,其油脂产量、油脂含量分别较出发菌株提高了17.59%和46.08%。对LA1发酵培养条件进行优化,得到最佳条件为初始葡萄糖质量浓度70 g/L,氮源为(NH4)2SO4,C/N值53。在最佳发酵条件下,LA1的油脂产量、生物量、油脂含量及油脂得率较优化前分别提高了123.9%、56.6%、43.0%和43.5%,其油脂脂肪酸组成与植物油相近。
이점홍효모( Rhodotorula glutinis)위출발균주,채용상압실온등리자체대기진행유변。득도최가유변조건위120 W공솔하처리60 s,치사솔체90%。유변후,채용소단흑B염색법사선출1주고산유지돌변주LA1,여출발균주상비교,기유지산량、유지함량분별교출발균주제고료17.59%화46.08%。대LA1발효배양조건진행우화,득도최가조건위초시포도당질량농도70 g/L,담원위(NH4)2SO4,C/N치53。재최가발효조건하,LA1적유지산량、생물량、유지함량급유지득솔교우화전분별제고료123.9%、56.6%、43.0%화43.5%,기유지지방산조성여식물유상근。
The Rhodotorula glutinis was mutagenized by atmospheric and room temperature plasma ( ARTP) . The optimal mutation condition was 60 s at 120 W,under this condition the lethality rate was 90%. The strain LA1 with high lipid production was screened from mutagenized Rhodotorula glutinis by Sudan black B staining method. The lipid productivity and lipid content of LA1 increased by 17. 59%and 46. 08% respectively comparing with Rhodotorula glutinis. The fermentation conditions of LA1 were optimized as follows:initial mass concentration of glucose 70 g/L, with (NH4 )2 SO4 as nitrogen source and C/N 53. Under these conditions, the lipid productivity, biomass, lipid content and lipid yield of LA1 in-creased by 123. 9%,56. 6%,43. 0% and 43. 5% respectively, and the fatty acid composition of the lipid of LA1 was similar to that of vegetable oil.